Literature summary for 1.14.13.22 extracted from

Bucko, M.; Schenkmayerova, A.; Gemeiner, P.; Vikartovska, A.; Mihovilovic, M.D.; Lacik, I.
Continuous testing system for Baeyer-Villiger biooxidation using recombinant Escherichia coli expressing cyclohexanone monooxygenase encapsulated in polyelectrolyte complex capsules (2011), Enzyme Microb. Technol., 49, 284-288.

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Application

Application	Comment	Organism
synthesis	model enantioselective Baeyer-Villiger biooxidations of rac-bicyclo[3.2.0]hept-2-en-6-one to corresponding lactones (1R,5S)-3-oxabicyclo-[3.3.0]oct-6-en-3-one and (1S,5R)-2-oxabicyclo-[3.3.0]oct-6-en-3-one as important chiral synthons for the synthesis of bioactive compounds. Reactions are performed in the minireactor equipped with a column packed with encapsulated recombinant cells Escherichia coli overexpressing cyclohexanone monooxygenase. The cells are encapsulated in polyelectrolyte complex capsules formed by reaction of oppositely charged polymers. Encapsulated cells tested in inireactor exhibit high operational stability with 4 complete substrate conversions to products and 6 conversions above 80% within 14 repeated consecutive biooxidation tests. Encapsulated cells show high enzyme stability during 91 days of storage with substrate conversions above 80% up to 60 days of storage	Acinetobacter calcoaceticus

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Acinetobacter calcoaceticus

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining

Organism

Organism	UniProt	Comment	Textmining
Acinetobacter calcoaceticus	-	-	-
Acinetobacter calcoaceticus NCIMB 9871	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
bicyclo[3.2.0]hept-2-en-6-one + NADPH + H+ + O2	-	Acinetobacter calcoaceticus	(1R,5S)-3-oxabicyclo-[3.3.0]oct-6-en-3-one + (1S,5R)-2-oxabicyclo-[3.3.0]oct-6-en-3-one + NADP+ + H2O	-	?
bicyclo[3.2.0]hept-2-en-6-one + NADPH + H+ + O2	-	Acinetobacter calcoaceticus NCIMB 9871	(1R,5S)-3-oxabicyclo-[3.3.0]oct-6-en-3-one + (1S,5R)-2-oxabicyclo-[3.3.0]oct-6-en-3-one + NADP+ + H2O	-	?

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Release 2023.1 (January 2023)