Application | Comment | Organism |
---|---|---|
synthesis | analysis of conversion kinetics for isolated enzyme, suspended whole cells, and biofilms, the latter two based on recombinant CHMO-containing P. taiwanensis VLB120. Biofilms show less favorable values for KS (9.3fold higher) and kcat (4.8fold lower) compared with corresponding KM and kcat values of isolated CHMO, but a favorable KI for cyclohexanone (5.3fold higher). Suspended cells show only 1.8fold higher KS, but 1.3- and 4.2fold higher kcat and KI values than isolated CHMO | Acidovorax sp. CHX100 |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0022 | - |
O2 | purified enzyme, pH not specified in the publication, temperature not specified in the publication | Acidovorax sp. CHX100 | |
0.054 | - |
O2 | suspended cells, pH not specified in the publication, temperature not specified in the publication | Acidovorax sp. CHX100 | |
0.178 | - |
cyclohexanone | purified enzyme, pH not specified in the publication, temperature not specified in the publication | Acidovorax sp. CHX100 | |
0.316 | - |
cyclohexanone | suspended cells, pH not specified in the publication, temperature not specified in the publication | Acidovorax sp. CHX100 | |
0.372 | - |
NADPH | purified enzyme, pH not specified in the publication, temperature not specified in the publication | Acidovorax sp. CHX100 | |
1.648 | - |
cyclohexanone | biofilm, pH not specified in the publication, temperature not specified in the publication | Acidovorax sp. CHX100 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acidovorax sp. CHX100 | A0A2D0WG32 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3,3,5-trimethylcyclohexanone + NADPH + O2 + H+ | 90% conversion efficincy, purified enzyme | Acidovorax sp. CHX100 | 4,6,6-trimethyloxepan-2-one + 4,4,6-trimethyloxepan-2-one + NADP+ + H2O | - |
? | |
4-ethylcyclohexanone + NADPH + O2 + H+ | 95% conversion efficincy, purified enzyme | Acidovorax sp. CHX100 | (5S)-5-ethyloxepan-2-one + NADP+ + H2O | - |
? | |
4-methylcyclohexanone + NADPH + O2 + H+ | 70% conversion efficincy, purified enzyme | Acidovorax sp. CHX100 | (5S)-5-methyloxepan-2-one + NADP+ + H2O | - |
? | |
beta-tetralonne + NADPH + H+ + O2 | 25% conversion efficincy, purified enzyme | Acidovorax sp. CHX100 | ? + NADP+ + H2O | - |
? | |
cyclohexanone + NADPH + H+ + O2 | 100% conversion efficincy, purified enzyme | Acidovorax sp. CHX100 | hexano-6-lactone + NADP+ + H2O | - |
? | |
cyclopentanone + NADPH + H+ + O2 | 100% conversion efficincy, purified enzyme | Acidovorax sp. CHX100 | 1-oxa-2-oxo-cyclohexane + NADP+ + H2O | - |
? | |
methyl phenyl sulfoxide + NADPH + H+ + O2 | 55% conversion efficincy, purified enzyme | Acidovorax sp. CHX100 | ? + NADP+ + H2O | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.24 | - |
cyclohexanone | biofilm, pH not specified in the publication, temperature not specified in the publication | Acidovorax sp. CHX100 | |
1.11 | - |
O2 | purified enzyme, pH not specified in the publication, temperature not specified in the publication | Acidovorax sp. CHX100 | |
1.16 | - |
cyclohexanone | purified enzyme, pH not specified in the publication, temperature not specified in the publication | Acidovorax sp. CHX100 | |
1.17 | - |
NADPH | purified enzyme, pH not specified in the publication, temperature not specified in the publication | Acidovorax sp. CHX100 | |
1.31 | - |
O2 | suspended cells, pH not specified in the publication, temperature not specified in the publication | Acidovorax sp. CHX100 | |
1.5 | - |
cyclohexanone | suspended cells, pH not specified in the publication, temperature not specified in the publication | Acidovorax sp. CHX100 |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.15 | - |
cyclohexanone | biofilm, pH not specified in the publication, temperature not specified in the publication | Acidovorax sp. CHX100 | |
0.28 | - |
O2 | suspended cells, pH not specified in the publication, temperature not specified in the publication | Acidovorax sp. CHX100 | |
3.16 | - |
NADPH | purified enzyme, pH not specified in the publication, temperature not specified in the publication | Acidovorax sp. CHX100 | |
4.75 | - |
cyclohexanone | suspended cells, pH not specified in the publication, temperature not specified in the publication | Acidovorax sp. CHX100 | |
5.79 | - |
O2 | purified enzyme, pH not specified in the publication, temperature not specified in the publication | Acidovorax sp. CHX100 | |
6.54 | - |
cyclohexanone | purified enzyme, pH not specified in the publication, temperature not specified in the publication | Acidovorax sp. CHX100 |