BRENDA - Enzyme Database
show all sequences of 1.14.13.220

gamma-Resorcylate catabolic-pathway genes in the soil actinomycete Rhodococcus jostii RHA1

Kasai, D.; Araki, N.; Motoi, K.; Yoshikawa, S.; Iino, T.; Imai, S.; Masai, E.; Fukuda, M.; Appl. Environ. Microbiol. 81, 7656-7665 (2015)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
expression in Escherichia coli BL21
Rhodococcus jostii
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
59000
-
1 * 59000, SDS-PAGE
Rhodococcus jostii
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
resorcinol + NADH + H+ + O2
Rhodococcus jostii
the enzyme gamma-resorcylate catabolic-pathway
hydroxyquinol + NAD+ + H2O
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Rhodococcus jostii
-
-
-
Purification (Commentary)
Purification (Commentary)
Organism
recombinant His-tagged protein
Rhodococcus jostii
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
resorcinol + NADH + H+ + O2
the enzyme gamma-resorcylate catabolic-pathway
735508
Rhodococcus jostii
hydroxyquinol + NAD+ + H2O
-
-
-
?
resorcinol + NADH + H+ + O2
resorcinol i.e. benzene-1,3-diol. The enzyme has no activity with NADPH
735508
Rhodococcus jostii
hydroxyquinol + NAD+ + H2O
hydroxyquinol i.e. benzene-1,2,4-triol
-
-
?
Subunits
Subunits
Commentary
Organism
monomer
1 * 59000, SDS-PAGE
Rhodococcus jostii
Synonyms
Synonyms
Commentary
Organism
tsdB
-
Rhodococcus jostii
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
30
-
assay at
Rhodococcus jostii
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
assay at
Rhodococcus jostii
Cofactor
Cofactor
Commentary
Organism
Structure
NADH
the enzyme has no activity with NADPH
Rhodococcus jostii
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli BL21
Rhodococcus jostii
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADH
the enzyme has no activity with NADPH
Rhodococcus jostii
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
59000
-
1 * 59000, SDS-PAGE
Rhodococcus jostii
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
resorcinol + NADH + H+ + O2
Rhodococcus jostii
the enzyme gamma-resorcylate catabolic-pathway
hydroxyquinol + NAD+ + H2O
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
recombinant His-tagged protein
Rhodococcus jostii
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
resorcinol + NADH + H+ + O2
the enzyme gamma-resorcylate catabolic-pathway
735508
Rhodococcus jostii
hydroxyquinol + NAD+ + H2O
-
-
-
?
resorcinol + NADH + H+ + O2
resorcinol i.e. benzene-1,3-diol. The enzyme has no activity with NADPH
735508
Rhodococcus jostii
hydroxyquinol + NAD+ + H2O
hydroxyquinol i.e. benzene-1,2,4-triol
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
monomer
1 * 59000, SDS-PAGE
Rhodococcus jostii
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
30
-
assay at
Rhodococcus jostii
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
assay at
Rhodococcus jostii
Expression
Organism
Commentary
Expression
Rhodococcus jostii
transcription of tsdB is activated when cells are grown on gamma-resorcylate
up
General Information
General Information
Commentary
Organism
metabolism
the enzyme gamma-resorcylate catabolic-pathway
Rhodococcus jostii
General Information (protein specific)
General Information
Commentary
Organism
metabolism
the enzyme gamma-resorcylate catabolic-pathway
Rhodococcus jostii
Expression (protein specific)
Organism
Commentary
Expression
Rhodococcus jostii
transcription of tsdB is activated when cells are grown on gamma-resorcylate
up
Other publictions for EC 1.14.13.220
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
735508
Kasai
gamma-Resorcylate catabolic-pa ...
Rhodococcus jostii
Appl. Environ. Microbiol.
81
7656-7665
2015
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