BRENDA - Enzyme Database show
show all sequences of 1.14.13.225

Kinetic and spectroscopic characterization of the putative monooxygenase domain of human MICAL-1

Zucchini, D.; Caprini, G.; Pasterkamp, R.J.; Tedeschi, G.; Vanoni, M.A.; Arch. Biochem. Biophys. 515, 1-13 (2011)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
F-actin
stimulates NADPH oxidation by increasing kcat and kcat/KNADPH about 5 and 200fold, respectively
Homo sapiens
Inhibitors
Inhibitors
Commentary
Organism
Structure
glycerol
10%, 2fold increase in Km for NADPH
Homo sapiens
NaCl
0.1 M, 20fold increase in Km for NADPH
Homo sapiens
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
NADPH
the Km for NADPH of the NADPH oxidase reaction is sensitive to ionic strength and type of ions. pH 7.0, 25°C
Homo sapiens
0.028
-
NADPH
pH 7.0, 25°C
Homo sapiens
0.093
-
NADPH
pH 7.0, 25°C, presence of 10% glycerol
Homo sapiens
0.499
-
NADPH
pH 7.0, 25°C, presence of 0.1 M NaCl
Homo sapiens
0.58
-
NADH
pH 7.0, 25°C
Homo sapiens
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Homo sapiens
Q8TDZ2
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
the Km for NADPH of the NADPH oxidase reaction is sensitive to ionic strength and type of ions. The apparent kcat (pH 7) is limited by enzyme reduction by NADPH, which occurs without detectable intermediates. The reaction is also sensitive to solvent viscosity
737550
Homo sapiens
?
-
-
-
-
[F-actin]-L-methionine + NADH + O2 + H+
-
737550
Homo sapiens
[F-actin]-L-methionine-(R)-S-oxide + NAD+ + H2O
-
-
-
?
[F-actin]-L-methionine + NADPH + O2 + H+
-
737550
Homo sapiens
[F-actin]-L-methionine-(R)-S-oxide + NADP+ + H2O
-
-
-
?
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.28
-
NADH
pH 7.0, 25°C
Homo sapiens
2.6
-
NADPH
pH 7.0, 25°C, presence of 0.1 M NaCl
Homo sapiens
2.9
-
NADPH
pH 7.0, 25°C, presence of 10% glycerol
Homo sapiens
4
-
NADPH
pH 7.0, 25°C
Homo sapiens
Cofactor
Cofactor
Commentary
Organism
Structure
NADH
NADPH is 10fold preferred over NADH
Homo sapiens
NADPH
10fold preferred over NADH
Homo sapiens
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
F-actin
stimulates NADPH oxidation by increasing kcat and kcat/KNADPH about 5 and 200fold, respectively
Homo sapiens
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADH
NADPH is 10fold preferred over NADH
Homo sapiens
NADPH
10fold preferred over NADH
Homo sapiens
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
glycerol
10%, 2fold increase in Km for NADPH
Homo sapiens
NaCl
0.1 M, 20fold increase in Km for NADPH
Homo sapiens
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
NADPH
the Km for NADPH of the NADPH oxidase reaction is sensitive to ionic strength and type of ions. pH 7.0, 25°C
Homo sapiens
0.028
-
NADPH
pH 7.0, 25°C
Homo sapiens
0.093
-
NADPH
pH 7.0, 25°C, presence of 10% glycerol
Homo sapiens
0.499
-
NADPH
pH 7.0, 25°C, presence of 0.1 M NaCl
Homo sapiens
0.58
-
NADH
pH 7.0, 25°C
Homo sapiens
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
the Km for NADPH of the NADPH oxidase reaction is sensitive to ionic strength and type of ions. The apparent kcat (pH 7) is limited by enzyme reduction by NADPH, which occurs without detectable intermediates. The reaction is also sensitive to solvent viscosity
737550
Homo sapiens
?
-
-
-
-
[F-actin]-L-methionine + NADH + O2 + H+
-
737550
Homo sapiens
[F-actin]-L-methionine-(R)-S-oxide + NAD+ + H2O
-
-
-
?
[F-actin]-L-methionine + NADPH + O2 + H+
-
737550
Homo sapiens
[F-actin]-L-methionine-(R)-S-oxide + NADP+ + H2O
-
-
-
?
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.28
-
NADH
pH 7.0, 25°C
Homo sapiens
2.6
-
NADPH
pH 7.0, 25°C, presence of 0.1 M NaCl
Homo sapiens
2.9
-
NADPH
pH 7.0, 25°C, presence of 10% glycerol
Homo sapiens
4
-
NADPH
pH 7.0, 25°C
Homo sapiens
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.48
-
NADH
pH 7.0, 25°C
Homo sapiens
5.2
-
NADPH
pH 7.0, 25°C, presence of 0.1 M NaCl
Homo sapiens
31.2
-
NADPH
pH 7.0, 25°C, presence of 10% glycerol
Homo sapiens
143
-
NADPH
pH 7.0, 25°C
Homo sapiens
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.48
-
NADH
pH 7.0, 25°C
Homo sapiens
5.2
-
NADPH
pH 7.0, 25°C, presence of 0.1 M NaCl
Homo sapiens
31.2
-
NADPH
pH 7.0, 25°C, presence of 10% glycerol
Homo sapiens
143
-
NADPH
pH 7.0, 25°C
Homo sapiens
Other publictions for EC 1.14.13.225
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
737558
Vitali
Properties and catalytic activ ...
Homo sapiens
Arch. Biochem. Biophys.
593
24-37
2016
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1
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5
1
1
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1
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1
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4
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1
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1
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1
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5
1
1
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1
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4
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4
4
739134
Grintsevich
F-actin dismantling through a ...
Drosophila melanogaster
Nat. Cell Biol.
18
876-885
2016
2
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1
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1
1
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739676
Alqassim
Modulation of MICAL monooxygen ...
Mus musculus
Sci. Rep.
6
22176
2016
-
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-
1
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1
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1
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1
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1
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737683
McDonald
Actin stimulates reduction of ...
Mus musculus
Biochemistry
52
6076-6084
2013
-
-
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1
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2
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2
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2
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2
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739131
Hung
SelR reverses Mical-mediated o ...
Drosophila melanogaster
Nat. Cell Biol.
15
1445-1454
2013
-
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1
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1
1
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737550
Zucchini
Kinetic and spectroscopic char ...
Homo sapiens
Arch. Biochem. Biophys.
515
1-13
2011
1
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2
5
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2
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2
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5
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3
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4
-
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4
4
739705
Hung
Direct redox regulation of F-a ...
Drosophila melanogaster
Science
334
1710-1713
2011
-
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-
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1
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1
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1
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1
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1
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1
1
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739160
Hung
Mical links semaphorins to F-a ...
Drosophila melanogaster
Nature
463
823-827
2010
-
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1
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1
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