Literature summary for 1.14.13.236 extracted from

Studts, J.M.; Mitchell, K.H.; Pikus, J.D.; McClay, K.; Steffan, R.J.; Fox, B.G.
Optimized expression and purification of toluene 4-monooxygenase hydroxylase (2000), Protein Expr. Purif., 20, 58-65.

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Application

Application	Comment	Organism
synthesis	by manipulation of vectors and gene inserts to eliminate adventitious catalytic turnover of NADH, up to 60fold increase in the volumetric yield of T4moH activity can be obtained from recombinant fermentations in Escherichia coli BL21(DE3)	Pseudomonas mendocina

Cloned(Commentary)

Cloned (Comment)	Organism
-	Pseudomonas mendocina

Organism

Organism	UniProt	Comment	Textmining
Pseudomonas mendocina	Q00456 and Q00457 and Q00460	Q00456, Q00457 an Q00460 are components TmoA, TmoB and TmoE of diiron hydroxylase T4MOH, respectively	-
Pseudomonas mendocina KR1	Q00456 and Q00457 and Q00460	Q00456, Q00457 an Q00460 are components TmoA, TmoB and TmoE of diiron hydroxylase T4MOH, respectively	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
1250	-	pH 7.5, 25°C	Pseudomonas mendocina

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
toluene + NADH + H+ + O2	-	Pseudomonas mendocina	4-methylphenol + NAD+ + H2O	-	?
toluene + NADH + H+ + O2	-	Pseudomonas mendocina KR1	4-methylphenol + NAD+ + H2O	-	?

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Release 2023.1 (January 2023)