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Literature summary for 1.14.13.238 extracted from

  • Green, J.; Large, P.
    Subcellular localization and properties of partially purified dimethylamine and trimethylamine mono-oxygenase activities in Candida utilis (1984), J. Gen. Microbiol., 130, 2577-2588 .
    View publication on PubMed
Search for more literature for 1.14.13.238

Inhibitors

Inhibitors Comment Organism Structure
2,2'-bipyridyl 12.7% inhibition at 0.5 mM Cyberlindnera jadinii
carbon monoxide complete inhibition at 30 s bubbling Cyberlindnera jadinii
Cuprizone 4.9% inhibition at 12.5 mM Cyberlindnera jadinii
EDTA 9.8% inhibition at 2.5 mM Cyberlindnera jadinii
hydroxylammonium chloride 99% inhibition at 0.5 mM Cyberlindnera jadinii
Lilly 53325 65.6% inhibition at 0.5 mM Cyberlindnera jadinii
mercaptoethanol 67.3% inhibition at 0.5 mM Cyberlindnera jadinii
additional information not inhibited by aminoacetonitrile, metyrapone, iodoacetamide, and N-ethylmaleimide Cyberlindnera jadinii
pargyline hydrochloride 15.7% inhibition at 1.16 mM Cyberlindnera jadinii
potassium cyanide 78.7% inhibition at 0.5 mM Cyberlindnera jadinii
Proadifen hydrochloride 71.7% inhibition at 0.5 mM Cyberlindnera jadinii
Sodium azide 14.6% inhibition at 5 mM Cyberlindnera jadinii
Sodium mersalyl 96.4% inhibition at 0.5 mM Cyberlindnera jadinii
sodium p-hydroxymercuribenzoate 94.1% inhibition at 0.2 mM Cyberlindnera jadinii
Tetramethylammonium chloride 20.7% inhibition at 5 mM Cyberlindnera jadinii
trans-2-phenylcyclopropylamine 60.6% inhibition at 0.25 mM Cyberlindnera jadinii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.000833
-
 diethylmethylamine with NADH as cosubstrate, at pH 7.0 and 25°C Cyberlindnera jadinii
0.00118
-
 trimethylamine with NADH as cosubstrate, at pH 7.0 and 25°C Cyberlindnera jadinii
0.004
-
 dimethylethylamine with NADH as cosubstrate, at pH 7.0 and 25°C Cyberlindnera jadinii
0.00444
-
 triethylamine with NADH as cosubstrate, at pH 7.0 and 25°C Cyberlindnera jadinii
0.0217
-
 Diethylamine with NADH as cosubstrate, at pH 7.0 and 25°C Cyberlindnera jadinii
0.0283
-
 dibutylamine with NADH as cosubstrate, at pH 7.0 and 25°C Cyberlindnera jadinii
0.0285
-
 NADH at pH 7.0 and 25°C Cyberlindnera jadinii
0.0294
-
 methylbutylamine with NADH as cosubstrate, at pH 7.0 and 25°C Cyberlindnera jadinii
0.0368
-
 Dimethylamine with NADH as cosubstrate, at pH 7.0 and 25°C Cyberlindnera jadinii
0.0704
-
 methyloctylamine with NADH as cosubstrate, at pH 7.0 and 25°C Cyberlindnera jadinii
0.08
-
 Methylpropylamine with NADH as cosubstrate, at pH 7.0 and 25°C Cyberlindnera jadinii
0.093
-
 methylethylamine with NADH as cosubstrate, at pH 7.0 and 25°C Cyberlindnera jadinii
0.125
-
 ethylbutylamine with NADH as cosubstrate, at pH 7.0 and 25°C Cyberlindnera jadinii
0.166
-
 NADPH at pH 7.0 and 25°C Cyberlindnera jadinii
0.278
-
 methylisopropylamine with NADH as cosubstrate, at pH 7.0 and 25°C Cyberlindnera jadinii
0.303
-
 dipropylamine with NADH as cosubstrate, at pH 7.0 and 25°C Cyberlindnera jadinii
0.654
-
 Methylethanolamine with NADH as cosubstrate, at pH 7.0 and 25°C Cyberlindnera jadinii
0.8
-
 propylbutylamine with NADH as cosubstrate, at pH 7.0 and 25°C Cyberlindnera jadinii
0.833
-
 ethylpropylamine with NADH as cosubstrate, at pH 7.0 and 25°C Cyberlindnera jadinii

Localization

Localization Comment Organism GeneOntology No. Textmining
smooth endoplasmic reticulum
-
 Cyberlindnera jadinii 5790
-

Organism

Organism UniProt Comment Textmining
Cyberlindnera jadinii
-
-
-
Cyberlindnera jadinii WY-3
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Sepharose 2B column chromatography and 4-aminobutyl-Sepharose 4B column chromatography Cyberlindnera jadinii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dibutylamine + NADH + H+ + O2
-
 Cyberlindnera jadinii butylamine + acetaldehyde + NAD+ + H2O
-
 ?
dibutylamine + NADH + H+ + O2
-
 Cyberlindnera jadinii WY-3 butylamine + acetaldehyde + NAD+ + H2O
-
 ?
diethylamine + NADH + H+ + O2
-
 Cyberlindnera jadinii ethylamine + acetaldehyde + NAD+ + H2O
-
 ?
diethylamine + NADH + H+ + O2
-
 Cyberlindnera jadinii WY-3 ethylamine + acetaldehyde + NAD+ + H2O
-
 ?
diethylmethylamine + NADH + H+ + O2
-
 Cyberlindnera jadinii ?
-
 ?
diethylmethylamine + NADH + H+ + O2
-
 Cyberlindnera jadinii WY-3 ?
-
 ?
dimethylamine + NADH + H+ + O2
-
 Cyberlindnera jadinii methylamine + formaldehyde + NAD+ + H2O
-
 ?
dimethylamine + NADH + H+ + O2
-
 Cyberlindnera jadinii WY-3 methylamine + formaldehyde + NAD+ + H2O
-
 ?
dimethylamine + NADPH + H+ + O2
-
 Cyberlindnera jadinii methylamine + formaldehyde + NADP+ + H2O
-
 ?
dimethylamine + NADPH + H+ + O2
-
 Cyberlindnera jadinii WY-3 methylamine + formaldehyde + NADP+ + H2O
-
 ?
dimethylethylamine + NADH + H+ + O2
-
 Cyberlindnera jadinii ?
-
 ?
dipropylamine + NADH + H+ + O2
-
 Cyberlindnera jadinii propylamine + acetaldehyde + NAD+ + H2O
-
 ?
ethylbutylamine + NADH + H+ + O2
-
 Cyberlindnera jadinii ?
-
 ?
ethylpropylamine + NADH + H+ + O2
-
 Cyberlindnera jadinii ?
-
 ?
methylbutylamine + NADH + H+ + O2
-
 Cyberlindnera jadinii ?
-
 ?
methylethanolamine + NADH + H+ + O2
-
 Cyberlindnera jadinii ?
-
 ?
methylethylamine + NADH + H+ + O2
-
 Cyberlindnera jadinii ?
-
 ?
methylisopropylamine + NADH + H+ + O2
-
 Cyberlindnera jadinii ?
-
 ?
methyloctylamine + NADH + H+ + O2
-
 Cyberlindnera jadinii ?
-
 ?
methylpropylamine + NADH + H+ + O2
-
 Cyberlindnera jadinii ?
-
 ?
propylbutylamine + NADH + H+ + O2
-
 Cyberlindnera jadinii ?
-
 ?
triethylamine + NADH + H+ + O2
-
 Cyberlindnera jadinii ?
-
 ?
trimethylamine + NADH + H+ + O2
-
 Cyberlindnera jadinii ?
-
 ?

Synonyms

Synonyms Comment Organism
dimethylamine mono-oxygenase
-
 Cyberlindnera jadinii

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
23
-
 the enzyme remains stable after 6 h at 23°C Cyberlindnera jadinii

Cofactor

Cofactor Comment Organism Structure
FAD
-
 Cyberlindnera jadinii
FMN
-
 Cyberlindnera jadinii
NADH NADH is the more efficient donor compared to NADPH Cyberlindnera jadinii
NADPH NADH is the more efficient donor compared to NADPH Cyberlindnera jadinii