Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.14.13.239 extracted from

  • Zhu, Y.; Jameson, E.; Crosatti, M.; Schaefer, H.; Rajakumar, K.; Bugg, T.D.; Chen, Y.
    Carnitine metabolism to trimethylamine by an unusual Rieske-type oxygenase from human microbiota (2014), Proc. Natl. Acad. Sci. USA, 111, 4268-4273 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.14.13.239

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BLR(DE3) pLysS cells Acinetobacter baumannii

Protein Variants

Protein Variants Comment Organism
E205A inactive Acinetobacter baumannii
E205D inactive Acinetobacter baumannii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-carnitine + NADH + H+ + O2 Acinetobacter baumannii
-
 (3R)-3-hydroxy-4-oxobutanoate + trimethylamine + NAD+ + H2O
-
 ?
L-carnitine + NADH + H+ + O2 Acinetobacter baumannii ATCC19606
-
 (3R)-3-hydroxy-4-oxobutanoate + trimethylamine + NAD+ + H2O
-
 ?
L-carnitine + NADPH + H+ + O2 Acinetobacter baumannii
-
 (3R)-3-hydroxy-4-oxobutanoate + trimethylamine + NADP+ + H2O
-
 ?
L-carnitine + NADPH + H+ + O2 Acinetobacter baumannii ATCC19606
-
 (3R)-3-hydroxy-4-oxobutanoate + trimethylamine + NADP+ + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Acinetobacter baumannii D0C9N6 AND D0C9N8 alpha subunit and beta subunit
-
Acinetobacter baumannii ATCC19606 D0C9N6 AND D0C9N8 alpha subunit and beta subunit
-

Purification (Commentary)

Purification (Comment) Organism
His-tag affinity column chromatography Acinetobacter baumannii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-carnitine + NADH + H+ + O2
-
 Acinetobacter baumannii (3R)-3-hydroxy-4-oxobutanoate + trimethylamine + NAD+ + H2O
-
 ?
L-carnitine + NADH + H+ + O2
-
 Acinetobacter baumannii ATCC19606 (3R)-3-hydroxy-4-oxobutanoate + trimethylamine + NAD+ + H2O
-
 ?
L-carnitine + NADPH + H+ + O2
-
 Acinetobacter baumannii (3R)-3-hydroxy-4-oxobutanoate + trimethylamine + NADP+ + H2O
-
 ?
L-carnitine + NADPH + H+ + O2
-
 Acinetobacter baumannii ATCC19606 (3R)-3-hydroxy-4-oxobutanoate + trimethylamine + NADP+ + H2O
-
 ?

Synonyms

Synonyms Comment Organism
cntAB
-
 Acinetobacter baumannii

Cofactor

Cofactor Comment Organism Structure
FAD
-
 Acinetobacter baumannii
NAD+
-
 Acinetobacter baumannii
NADH
-
 Acinetobacter baumannii
NADPH
-
 Acinetobacter baumannii
[2Fe-2S]-center
-
 Acinetobacter baumannii