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Literature summary for 1.14.13.24 extracted from
- Purification and characterization of the 3-hydroxybenzoate-6-hydroxylase from Klebsiella pneumoniae (1995), FEMS Microbiol. Lett., 126, 283-290.
General Stability
| General Stability | Organism |
|---|---|
| complete inactivation at room temperature without glycerol after 18 h | Klebsiella pneumoniae |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | inhibition at 0.02 mM, partially reversed by 2-mercaptoethanol | Klebsiella pneumoniae |  |
| Fe2+ | inhibition at 0.02 mM, partially reversed by 2-mercaptoethanol | Klebsiella pneumoniae | |
| Hg2+ | inhibition at 0.02 mM, partially reversed by 2-mercaptoethanol | Klebsiella pneumoniae | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.07 | - |
NADPH | - |
Klebsiella pneumoniae | |
| 0.07 | - |
NADH | - |
Klebsiella pneumoniae | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 41500 | - |
gel filtration | Klebsiella pneumoniae |
| 42000 | - |
1 * 42000, SDS-PAGE | Klebsiella pneumoniae |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Klebsiella pneumoniae | - |
- |
- |
| Klebsiella pneumoniae M5a1 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| homogeneity | Klebsiella pneumoniae |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 8.42 | - |
- |
Klebsiella pneumoniae |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, optimal storage in 50 mM phosphate or Tris buffer pH 8 with 10% glycerol | Klebsiella pneumoniae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3-hydroxybenzoate + NADH + O2 | - |
Klebsiella pneumoniae | 2,5-dihydroxybenzoate + NAD+ + H2O | - |
? | |
| 3-hydroxybenzoate + NADH + O2 | - |
Klebsiella pneumoniae M5a1 | 2,5-dihydroxybenzoate + NAD+ + H2O | - |
? | |
| 3-hydroxybenzoate + NADPH + H+ + O2 | - |
Klebsiella pneumoniae | 2,5-dihydroxybenzoate + NADP+ + H2O | - |
r | |
| 3-hydroxybenzoate + NADPH + H+ + O2 | - |
Klebsiella pneumoniae M5a1 | 2,5-dihydroxybenzoate + NADP+ + H2O | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 42000, SDS-PAGE | Klebsiella pneumoniae |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | 30 | - |
Klebsiella pneumoniae |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 15 | 40 | no activity below 15°C and above 40°C | Klebsiella pneumoniae |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Klebsiella pneumoniae |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 11 | no activity below pH 6.5 and above pH 11 | Klebsiella pneumoniae |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 8.5 | in phosphate and Tris-HCl buffer | Klebsiella pneumoniae |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | - |
Klebsiella pneumoniae | |
| NADPH | NADH and NADPH utilized with similar efficiencies | Klebsiella pneumoniae | |
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