Any feedback?
Literature summary for 1.14.13.24 extracted from
- Reduction kinetics of 3-hydroxybenzoate 6-hydroxylase from Rhodococcus jostii RHA1 (2012), Biochemistry, 51, 4309-4321.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli Top10 cells | Rhodococcus jostii |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 47000 | - |
2 * 47000 | Rhodococcus jostii |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3-hydroxybenzoate + NADH + H+ + O2 | Rhodococcus jostii | - |
2,5-dihydroxybenzoate + NAD+ + H2O | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rhodococcus jostii | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| DEAE-Sepharose column chromatography and Sephadex G-25 gel filtration | Rhodococcus jostii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3-hydroxybenzoate + NADH + H+ + O2 | - |
Rhodococcus jostii | 2,5-dihydroxybenzoate + NAD+ + H2O | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homodimer | 2 * 47000 | Rhodococcus jostii |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| 3-hydroxybenzoate 6-hydroxylase | - |
Rhodococcus jostii |
| 3HB6H | - |
Rhodococcus jostii |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | the enzyme contains one FAD per subunit | Rhodococcus jostii | |
| NADH | - |
Rhodococcus jostii | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
