Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.14.13.24 extracted from

  • Westphal, A.H.; Tischler, D.; van Berkel, W.J.H.
    Natural diversity of FAD-dependent 4-hydroxybenzoate hydroxylases (2021), Arch. Biochem. Biophys., 702, 108820 .
    View publication on PubMed
Search for more literature for 1.14.13.24

Application

Application Comment Organism
analysis 3-hydroxybenzoate 6-hydroxylase (3HB6H, EC 1.14.13.24) from Rhodococcus jostii RHA1 is used as a lipid-containing reference protein for enzyme 4-hydroxybenzoate 1-hydroxylase, 4HB1H (EC 1.14.13.64). Models for Cp_4HB1H from Candida parapsilosis strain CBS604, Fo_4HB1H-like from Fusarium oxysporum, and VibMO1 from Boreostereum vibrans (UniProt ID A0A167KUL3) are generated using Rhodococcus jostii Rj_3HB6H (PDB ID 4bk1) and Pseudomonas putida Pp_SALH (PDB ID 5evy) as template structures, overview Rhodococcus jostii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3-hydroxybenzoate + NADH + H+ + O2 Rhodococcus jostii
-
 2,5-dihydroxybenzoate + NAD+ + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Rhodococcus jostii
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
lipoprotein the phospholipid has been proposed to be important for orienting the 3-HB substrate of 3HB6H for regioselective hydroxylation at C6 Rhodococcus jostii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-hydroxybenzoate + NADH + H+ + O2
-
 Rhodococcus jostii 2,5-dihydroxybenzoate + NAD+ + H2O
-
 ?

Synonyms

Synonyms Comment Organism
3-hydroxybenzoate 6-hydroxylase
-
 Rhodococcus jostii
3HB6H
-
 Rhodococcus jostii

Cofactor

Cofactor Comment Organism Structure
FAD
-
 Rhodococcus jostii
NADH
-
 Rhodococcus jostii

General Information

General Information Comment Organism
evolution evolutionary relationship between the NAD(P)H-dependent FAD-containing 4-hydroxybenzoate hydroxylases and phylogenetic analysis of group A FPMOs, overview Rhodococcus jostii
additional information 3-hydroxybenzoate 6-hydroxylase (3HB6H) structure analysis and comparison with structures of several FAD-dependent 4-hydroxybenzoate hydroxylases, homology modeling, detailed overview. FAD-dependent hydroxybenzoate hydroxylase enzymes structure comparisons Rhodococcus jostii