Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1-deaza-FAD | - |
Pseudomonas sp. | |
5-pyridoxic acid | competitive | Pseudomonas sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
43000 | - |
4 * 43000, SDS-PAGE | Pseudomonas sp. |
160000 | - |
equilibrium sedimentation | Pseudomonas sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas sp. | - |
- |
- |
Oxidation Stability | Organism |
---|---|
the enzyme is very sensitive to oxidation, it loses activity rapidly in absence of mercaptoethanol even at 4°C, it is further stabilized in presence of high concentrations of glycerol or by serum albumin | Pseudomonas sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-hydroxy-2-methylpyridine-5-carboxylate + NADH + O2 | - |
Pseudomonas sp. | 2-(acetamidomethylene)succinate + NAD(P)+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 43000, SDS-PAGE | Pseudomonas sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | contains 2 mol of FAD per mol of tetrameric enzyme. 412 nM | Pseudomonas sp. | |
NADH | interacts with the holoenzyme in a slow catalytically irrelevant manner | Pseudomonas sp. |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00046 | - |
1-deaza-FAD | - |
Pseudomonas sp. | |
0.023 | - |
5-pyridoxic acid | - |
Pseudomonas sp. |