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Literature summary for 1.14.13.242 extracted from

  • Yuan, B.; Yokochi, N.; Yoshikane, Y.; Ohnishi, K.; Yagi, T.
    Molecular cloning, identification and characterization of 2-methyl-3-hydroxypyridine-5-carboxylic-acid-dioxygenase-coding gene from the nitrogen-fixing symbiotic bacterium Mesorhizobium loti (2006), J. Biosci. Bioeng., 102, 504-510 .
    View publication on PubMed
Search for more literature for 1.14.13.242

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Mesorhizobium loti

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0254
-
 3-Hydroxy-2-methylpyridine-5-carboxylate pH 8.0, 25°C Mesorhizobium loti
0.0586
-
 O2 pH 8.0, 25°C Mesorhizobium loti
0.0589
-
 5-hydroxynicotinic acid pH 8.0, 25°C Mesorhizobium loti
0.0779
-
 NADH pH 8.0, 25°C Mesorhizobium loti

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
164000
-
 gel fitlration Mesorhizobium loti

Organism

Organism UniProt Comment Textmining
Mesorhizobium loti Q988D3
-
-
Mesorhizobium loti MAFF 303099 Q988D3
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.28
-
 pH 8.0, 25°C Mesorhizobium loti

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-hydroxy-2-methylpyridine-5-carboxylate + NADH + O2 + H+
-
 Mesorhizobium loti 2-(acetamidomethylene)succinate + NAD+
-
 ?
3-hydroxy-2-methylpyridine-5-carboxylate + NADH + O2 + H+
-
 Mesorhizobium loti MAFF 303099 2-(acetamidomethylene)succinate + NAD+
-
 ?
5-hydroxynicotinic acid + NADH + O2 + H+
-
 Mesorhizobium loti 2-(formylamidomethylene)succinate + NAD+
-
 ?
5-hydroxynicotinic acid + NADH + O2 + H+
-
 Mesorhizobium loti MAFF 303099 2-(formylamidomethylene)succinate + NAD+
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 42000, SDS-PAGE, 4 * 41747, calculated Mesorhizobium loti

Synonyms

Synonyms Comment Organism
MHPCO
-
 Mesorhizobium loti
mlr6788
-
 Mesorhizobium loti

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
 Mesorhizobium loti

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20
-
 79% of maximum activity Mesorhizobium loti
40
-
 82% of maximum activity Mesorhizobium loti

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
 stable for 10 min Mesorhizobium loti

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
10.7
-
 5-hydroxynicotinic acid pH 8.0, 25°C Mesorhizobium loti
11.8
-
 3-Hydroxy-2-methylpyridine-5-carboxylate pH 8.0, 25°C Mesorhizobium loti

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 substrate 3-hydroxy-2-methylpyridine-5-carboxylate Mesorhizobium loti
8
-
 substrate 5-hydroxynicotinic acid Mesorhizobium loti

Cofactor

Cofactor Comment Organism Structure
FAD Km value 624 nM, one mol of the enzyme contains 1.93 mol of FAD Mesorhizobium loti
NADH
-
 Mesorhizobium loti

General Information

General Information Comment Organism
physiological function MHPCO is essential for the assimilation of pyridoxine, but not for its growth in a nutrient-rich medium. MHPCO is dispensable for at least nodule formation on roots of seedlings in symbiosis Mesorhizobium loti