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Literature summary for 1.14.13.25 extracted from

  • Liu, A.; Jin, Y.; Zhang, J.; Brazeau, B.J.; Lipscomb, J.D.
    Substrate radical intermediates in soluble methane monooxygenase (2005), Biochem. Biophys. Res. Commun., 338, 254-261.
    View publication on PubMed
Search for more literature for 1.14.13.25

Localization

Localization Comment Organism GeneOntology No. Textmining
soluble
-
 Methylosinus trichosporium
-
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
methane + NADH + O2 Methylosinus trichosporium
-
 methanol + NAD+ + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Methylosinus trichosporium
-
 a type II methanotroph
-

Reaction

Reaction Comment Organism Reaction ID
methane + NAD(P)H + H+ + O2 = methanol + NAD(P)+ + H2O reaction mechanism, substrate radical intermediates in the reaction of soluble methane monooxygenase, the reaction involves the conversion of the enzyme hydroxylase component, MMOH, diiron cluster to a strongly oxidizing bis-l-oxo-Fe(IV)2 species termed compound Q, that is capable of the fissure of stable C–H bonds in methane and many other hydrocarbons Methylosinus trichosporium
a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 effects of spin-traps on MMO activity, spin trapping by 5,5-dimethyl-1-pyrroline N-oxide and alpha-4-pyridyl-1-oxide N-tert-butylnitrone, mechanism, overview Methylosinus trichosporium

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,1,2,2-tetramethylcyclopropane + NADH + O2
-
 Methylosinus trichosporium ?
-
 ?
ethane + NADH + O2
-
 Methylosinus trichosporium ?
-
 ?
methane + NADH + O2
-
 Methylosinus trichosporium methanol + NAD+ + H2O
-
 ?
additional information effects of spin-traps on MMO activity, overview Methylosinus trichosporium ?
-
 ?
nitrobenzene + NADH + O2 an electron is removed from nitrobenzene by Q in the first step of the reaction and then the bound hydroxyl radical formed in this process rebounds to form nitrophenol Methylosinus trichosporium nitrophenol + NAD+ + H2O
-
 ?

Synonyms

Synonyms Comment Organism
MMO
-
 Methylosinus trichosporium

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
23
-
 assay at Methylosinus trichosporium

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.6
-
 assay at Methylosinus trichosporium

Cofactor

Cofactor Comment Organism Structure
NADH
-
 Methylosinus trichosporium