Application | Comment | Organism |
---|---|---|
biotechnology | high particulate methane monooxygenase activity may contribute to the synthesis of poly-beta-hydroxybutyrate in the cell, which may be used to improve the yield of poly-beta-hydroxybutyrate in methanotrophs | Methylococcus capsulatus |
biotechnology | high particulate methane monooxygenase activity may contribute to the synthesis of poly-beta-hydroxybutyrate in the cell, which may be used to improve the yield of poly-beta-hydroxybutyrate in methanotrophs | Methylomonas sp. GYJ3 |
biotechnology | high particulate methane monooxygenase activity may contribute to the synthesis of poly-beta-hydroxybutyrate in the cell, which may be used to improve the yield of poly-beta-hydroxybutyrate in methanotrophs. Poly-beta-hydroxybutyrate content of strain OB3b can reach the highest level in the shortest time as compared to other methanotrophs. Nutrients deficiency condition is beneficial for strain IMV3011 to synthesize PHB whereas it is not beneficial for other strains | Methylosinus trichosporium |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | when cultivated in nutrients deficiency culture, activity of sMMO does not decrease | Methylococcus capsulatus | |
additional information | when cultivated in nutrients deficiency culture, activity of sMMO decreases | Methylomonas sp. GYJ3 | |
additional information | when cultivated in nutrients deficiency culture, activity of sMMO in strain OB3b decreases, whereas activity of sMMO in strain IMV3011 does not decrease | Methylosinus trichosporium | |
poly-beta-hydroxybutyrate | MMO activity dramatically decreases when cellular poly-beta-hydroxybutyrate accumulates in the second stage. The more poly-beta-hydroxybutyrate are accumulated, the slower MMO activity decreases. Cellular poly-beta-hydroxybutyrate content has some influence on the maintenance of the MMO activity | Methylococcus capsulatus | |
poly-beta-hydroxybutyrate | MMO activity dramatically decreases when cellular poly-beta-hydroxybutyrate accumulates in the second stage. The more poly-beta-hydroxybutyrate are accumulated, the slower MMO activity decreases. Cellular poly-beta-hydroxybutyrate content has some influence on the maintenance of the MMO activity | Methylomonas sp. GYJ3 | |
poly-beta-hydroxybutyrate | MMO activity dramatically decreases when cellular poly-beta-hydroxybutyrate accumulates in the second stage. The more poly-beta-hydroxybutyrate are accumulated, the slower MMO activity decreases. Cellular poly-beta-hydroxybutyrate content has some influence on the maintenance of the MMO activity | Methylosinus trichosporium |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Methylococcus capsulatus | 5737 | - |
cytoplasm | - |
Methylosinus trichosporium | 5737 | - |
cytoplasm | - |
Methylomonas sp. | 5737 | - |
cytoplasm | sMMO | Methylococcus capsulatus | 5737 | - |
cytoplasm | sMMO | Methylosinus trichosporium | 5737 | - |
cytoplasm | sMMO | Methylomonas sp. GYJ3 | 5737 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | cells adapted to the respective medium, either lacking Cu (sMMO production) or containing 0.01 mM Cu (pMMO production) | Methylococcus capsulatus | |
Cu2+ | cells adapted to the respective medium, either lacking Cu (sMMO production) or containing 0.01 mM Cu (pMMO production) | Methylosinus trichosporium | |
Cu2+ | cells adapted to the respective medium, either lacking Cu (sMMO production) or containing 0.01 mM Cu (pMMO production) | Methylomonas sp. GYJ3 | |
additional information | sMMO activity and expression does not require Cu2+ | Methylococcus capsulatus | |
additional information | sMMO activity and expression does not require Cu2+ | Methylosinus trichosporium | |
additional information | sMMO activity and expression does not require Cu2+ | Methylomonas sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methylococcus capsulatus | - |
- |
- |
Methylococcus capsulatus | - |
HD6T | - |
Methylococcus capsulatus HD6T | - |
- |
- |
Methylococcus capsulatus HD6T | - |
HD6T | - |
Methylomonas sp. | - |
- |
- |
Methylomonas sp. GYJ3 | - |
- |
- |
Methylosinus trichosporium | - |
- |
- |
Methylosinus trichosporium | - |
strains OB3b and IMV3011 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
methane + NADH + H+ + O2 | - |
Methylococcus capsulatus | methanol + NAD+ + H2O | - |
? | |
methane + NADH + H+ + O2 | - |
Methylosinus trichosporium | methanol + NAD+ + H2O | - |
? | |
methane + NADH + H+ + O2 | - |
Methylomonas sp. | methanol + NAD+ + H2O | - |
? | |
methane + NADH + H+ + O2 | - |
Methylococcus capsulatus HD6T | methanol + NAD+ + H2O | - |
? | |
additional information | pMMO has broader substrate specificity but lower activity with smaller hydrocarbons like methane, ethane, and propene compared to pMMO | Methylococcus capsulatus | ? | - |
? | |
additional information | pMMO has broader substrate specificity but lower activity with smaller hydrocarbons like methane, ethane, and propene compared to pMMO | Methylosinus trichosporium | ? | - |
? | |
additional information | pMMO has broader substrate specificity but lower activity with smaller hydrocarbons like methane, ethane, and propene compared to pMMO | Methylomonas sp. | ? | - |
? | |
additional information | pMMO has broader substrate specificity but lower activity with smaller hydrocarbons like methane, ethane, and propene compared to pMMO | Methylococcus capsulatus HD6T | ? | - |
? | |
naphthalene + NAD(P)H + O2 | oxidized by sMMO | Methylococcus capsulatus | alpha-naphthol + beta-naphthol + NAD(P)+ + H2O | - |
? | |
naphthalene + NAD(P)H + O2 | oxidized by sMMO | Methylosinus trichosporium | alpha-naphthol + beta-naphthol + NAD(P)+ + H2O | - |
? | |
naphthalene + NAD(P)H + O2 | oxidized by sMMO | Methylomonas sp. GYJ3 | alpha-naphthol + beta-naphthol + NAD(P)+ + H2O | - |
? | |
naphthalene + NAD(P)H + O2 | oxidized by sMMO | Methylococcus capsulatus HD6T | alpha-naphthol + beta-naphthol + NAD(P)+ + H2O | - |
? | |
naphthalene + NADH + H+ | - |
Methylococcus capsulatus | alpha-naphthol + beta-naphthol + NAD+ + H2O | - |
? | |
naphthalene + NADH + H+ | - |
Methylosinus trichosporium | alpha-naphthol + beta-naphthol + NAD+ + H2O | - |
? | |
naphthalene + NADH + H+ | - |
Methylomonas sp. | alpha-naphthol + beta-naphthol + NAD+ + H2O | - |
? | |
naphthalene + NADH + H+ | - |
Methylococcus capsulatus HD6T | alpha-naphthol + beta-naphthol + NAD+ + H2O | - |
? | |
propene + NAD(P)H + O2 | - |
Methylococcus capsulatus | 1,2-epoxypropane + NAD(P)+ + H2O | - |
? | |
propene + NAD(P)H + O2 | - |
Methylosinus trichosporium | 1,2-epoxypropane + NAD(P)+ + H2O | - |
? | |
propene + NAD(P)H + O2 | - |
Methylomonas sp. GYJ3 | 1,2-epoxypropane + NAD(P)+ + H2O | - |
? | |
propene + NAD(P)H + O2 | - |
Methylococcus capsulatus HD6T | 1,2-epoxypropane + NAD(P)+ + H2O | - |
? | |
propene + NADH + H+ + O2 | - |
Methylococcus capsulatus | epoxypropane + NAD+ + H2O | - |
? | |
propene + NADH + H+ + O2 | - |
Methylosinus trichosporium | epoxypropane + NAD+ + H2O | - |
? | |
propene + NADH + H+ + O2 | - |
Methylomonas sp. | epoxypropane + NAD+ + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
MMO | - |
Methylococcus capsulatus |
MMO | - |
Methylosinus trichosporium |
MMO | - |
Methylomonas sp. GYJ3 |
particulate methane monooxygenase | - |
Methylococcus capsulatus |
particulate methane monooxygenase | - |
Methylosinus trichosporium |
particulate methane monooxygenase | - |
Methylomonas sp. GYJ3 |
pMMO | - |
Methylococcus capsulatus |
pMMO | - |
Methylosinus trichosporium |
pMMO | - |
Methylomonas sp. GYJ3 |
sMMO | - |
Methylococcus capsulatus |
sMMO | - |
Methylosinus trichosporium |
sMMO | - |
Methylomonas sp. |
sMMO | - |
Methylomonas sp. GYJ3 |
soluble methane monooxygenase | - |
Methylococcus capsulatus |
soluble methane monooxygenase | - |
Methylosinus trichosporium |
soluble methane monooxygenase | - |
Methylomonas sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | - |
Methylococcus capsulatus | |
NADH | - |
Methylosinus trichosporium | |
NADH | - |
Methylomonas sp. GYJ3 | |
NADPH | - |
Methylococcus capsulatus | |
NADPH | - |
Methylosinus trichosporium | |
NADPH | - |
Methylomonas sp. GYJ3 |
Organism | Comment | Expression |
---|---|---|
Methylococcus capsulatus | sMMO is expressed at low copper/biomass ratios | up |
Methylosinus trichosporium | sMMO is expressed at low copper/biomass ratios | up |
Methylomonas sp. | sMMO is expressed at low copper/biomass ratios | up |