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Literature summary for 1.14.13.25 extracted from

  • Yu, Y.; Ramsay, J.A.; Ramsay, B.A.
    Production of soluble methane monooxygenase during growth of Methylosinus trichosporium on methanol (2009), J. Biotechnol., 139, 78-83.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
additional information when the growth-limiting substrate is switched from methane to methanol, in the presence of the particulate MMO inhibitor allylthiourea, growth continues unabated and sMMO activity is completely retained. During the brief period when both methane and methanol are present, higher sMMO activity is observed Methylosinus trichosporium OB3b

Application

Application Comment Organism
biotechnology methanol can be employed to produce large amounts of Methylosinus trichosporium biomass containing sMMO. Enzyme expression can be maintained during growth on methanol which may aid in the development of sMMO-based industrial and environmental processes Methylosinus trichosporium OB3b

Metals/Ions

Metals/Ions Comment Organism Structure
Cu2+ when allylthiourea is removed, sMMO activity is maintained for an additional 24 generations, albeit at a slightly lower level due to the presence of 0.0007 mM of Cu2+ in the feed medium Methylosinus trichosporium OB3b

Organism

Organism UniProt Comment Textmining
Methylosinus trichosporium OB3b
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
methane + NAD(P)H + O2
-
Methylosinus trichosporium OB3b methanol + NAD(P)+ + H2O
-
?
naphthalene + NAD(P)H + O2
-
Methylosinus trichosporium OB3b alpha-naphthol + beta-naphthol + NAD(P)+ + H2O
-
?

Synonyms

Synonyms Comment Organism
sMMO
-
Methylosinus trichosporium OB3b
soluble methane monooxygenase
-
Methylosinus trichosporium OB3b

Cofactor

Cofactor Comment Organism Structure
NADH
-
Methylosinus trichosporium OB3b
NADPH
-
Methylosinus trichosporium OB3b