Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Streptomyces achromogenes subsp. streptozoticus |
Crystallization (Comment) | Organism |
---|---|
structure of the selenomethionine derivative, to 2.08 A resolution shows a dimer with three distinct domains | Streptomyces achromogenes subsp. streptozoticus |
Protein Variants | Comment | Organism |
---|---|---|
D315A | complete loss of activity | Streptomyces achromogenes subsp. streptozoticus |
E215A | complete loss of activity | Streptomyces achromogenes subsp. streptozoticus |
E281A | complete loss of activity | Streptomyces achromogenes subsp. streptozoticus |
H225A | complete loss of activity | Streptomyces achromogenes subsp. streptozoticus |
H311A | complete loss of activity | Streptomyces achromogenes subsp. streptozoticus |
H318A | complete loss of activity | Streptomyces achromogenes subsp. streptozoticus |
H407A/H409A/H448A | variant accumulates intermediate Ndelta,Nomega-dihydroxy-Nomega-methyl-L-arginine but does not generate the final product | Streptomyces achromogenes subsp. streptozoticus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron | recombinant protein purified aerobically contains around 0.9 Fe per monomer | Streptomyces achromogenes subsp. streptozoticus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Streptomyces achromogenes subsp. streptozoticus | SznF activity requires FeII, oxygen and external reductants. L-arginine is not accepted as a substrate | ? | - |
- |
|
Ndelta,Nomega-dihydroxy-Nomega-methyl-L-arginine + O2 | Streptomyces achromogenes subsp. streptozoticus | - |
Ndelta-hydroxy-Nomega-methyl-Nomega-nitroso-L-citrulline + H2O | - |
? | |
Ndelta-hydroxy-Nomega-methyl-L-arginine + NADH + H+ + O2 | Streptomyces achromogenes subsp. streptozoticus | - |
Ndelta,Nomega-dihydroxy-Nomega-methyl-L-arginine + NAD+ + H2O | - |
? | |
Nomega-methyl-L-arginine + 2 NADH + 2 H+ + 3 O2 | Streptomyces achromogenes subsp. streptozoticus | - |
Ndelta-hydroxy-Nomega-methyl-Nomega-nitroso-L-citrulline + 2 NAD+ + 3 H2O | - |
? | |
Nomega-methyl-L-arginine + NADH + H+ + O2 | Streptomyces achromogenes subsp. streptozoticus | - |
Ndelta-hydroxy-Nomega-methyl-L-arginine + NAD+ + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces achromogenes subsp. streptozoticus | A0A411MR89 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | SznF activity requires FeII, oxygen and external reductants. L-arginine is not accepted as a substrate | Streptomyces achromogenes subsp. streptozoticus | ? | - |
- |
|
Ndelta,Nomega-dihydroxy-Nomega-methyl-L-arginine + O2 | - |
Streptomyces achromogenes subsp. streptozoticus | Ndelta-hydroxy-Nomega-methyl-Nomega-nitroso-L-citrulline + H2O | - |
? | |
Ndelta-hydroxy-Nomega-methyl-L-arginine + NADH + H+ + O2 | - |
Streptomyces achromogenes subsp. streptozoticus | Ndelta,Nomega-dihydroxy-Nomega-methyl-L-arginine + NAD+ + H2O | - |
? | |
Nomega-methyl-L-arginine + 2 NADH + 2 H+ + 3 O2 | - |
Streptomyces achromogenes subsp. streptozoticus | Ndelta-hydroxy-Nomega-methyl-Nomega-nitroso-L-citrulline + 2 NAD+ + 3 H2O | - |
? | |
Nomega-methyl-L-arginine + NADH + H+ + O2 | - |
Streptomyces achromogenes subsp. streptozoticus | Ndelta-hydroxy-Nomega-methyl-L-arginine + NAD+ + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
StzF | - |
Streptomyces achromogenes subsp. streptozoticus |
sznF | - |
Streptomyces achromogenes subsp. streptozoticus |
General Information | Comment | Organism |
---|---|---|
metabolism | SznF catalyzes the oxidative rearrangement of a guanidine group to an N-nitrosourea in a reaction dependent on both non-haem iron and oxygen. The two metallocofactors of SznF are functionally distinct, with the central domain catalyzing two sequential N-hydroxylations of L-N-methylarginine and the cupin domain enabling oxidative rearrangement and N-N bond formation to yield the N-nitrosourea product | Streptomyces achromogenes subsp. streptozoticus |
physiological function | sznF is involved in the biosynthesis of streptozotocin, SznF catalyzes an oxidative rearrangement of the guanidine group of Nomega-methyl-L-arginine to generate an N-nitrosourea product using two separate active sites that promote distinct steps in this transformation using different iron-containing metallocofactors. Knockout of SznF abolishes the production of streptozotocin | Streptomyces achromogenes subsp. streptozoticus |