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Literature summary for 1.14.13.29 extracted from
- Modulation of porcine cytochrome P450 enzyme activities by surgical castration and immunocastration (2009), Animal, 3, 1124-1132.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| additional information | no inhibitory effects of testosterone (4, 8, 17, 34 pmol/ml), 17beta-estradiol (18, 3.6, 36, 72 pmol/ml), estrone (1.9, 3.7, 9, 18 pmol/ml), androstenone (55, 180, 3760, 7520 pmol/ml) | Sus scrofa |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| microsome | - |
Sus scrofa | - |
- |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| p-nitrophenol + NADPH + H+ + O2 | Sus scrofa | 200 microM substrate, 0.4 mg microsomal protein, 1 mM NADPH | p-nitrocatechol + NADP+ + H2O | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Sus scrofa | - |
male pigs, surgically castrated pigs, immunocastrated pigs | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| liver is homogenized, centrifuged, supernatant mixed with 8 mM calcium chloride, microsomes separated by centrifugation, pellet resuspended in 50 mM Tris-HCl with 0.1 mM ethylendiaminetetraacetic acid and 20% glycerol, pH 7.4 | Sus scrofa |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Sus scrofa | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 98.3 | - |
entire male pig, pH 6.8 | Sus scrofa |
| 132.8 | - |
surgically castrated pig, pH 6.8 | Sus scrofa |
| 161.5 | - |
immunocastrated pig, pH 6.8 | Sus scrofa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| p-nitrophenol + NADPH + H+ + O2 | 200 microM substrate, 0.4 mg microsomal protein, 1 mM NADPH | Sus scrofa | p-nitrocatechol + NADP+ + H2O | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CYP2A/2E1 | - |
Sus scrofa |
| p-nitrophenol hydroxylase | - |
Sus scrofa |
| PNPH | - |
Sus scrofa |
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