BRENDA - Enzyme Database show
show all sequences of 1.14.13.32

Chiral sulfoxidation of albendazole by the flavin adenine dinucleotide-containing and cytochrome P450-dependent monooxygenases from rat liver microsomes

Moroni, P.; Buronfosse, T.; Longin-Sauvageon, C.; Delatour, P.; Benoit, E.; Drug Metab. Dispos. 23, 160-165 (1995)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
Methimazole
competitive inhibitor, thermal treatment of the microsomes inhibited the methimazole oxidation
Rattus norvegicus
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
endoplasmic reticulum
-
Rattus norvegicus
5783
-
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
59000
-
SDS-PAGE
Rattus norvegicus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
albendazole + NADPH + O2
Rattus norvegicus
-
albendazole S-oxide + NADP+ + H2O
-
-
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Rattus norvegicus
-
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
liver
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.013
-
-
Rattus norvegicus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
albendazole + NADPH + O2
-
285316
Rattus norvegicus
albendazole S-oxide + NADP+ + H2O
-
-
-
-
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
additional information
-
2 min. 50°C in assay buffer in the absence of NADPH inactivates the enzyme
Rattus norvegicus
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.4
-
assay conditions
Rattus norvegicus
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Methimazole
competitive inhibitor, thermal treatment of the microsomes inhibited the methimazole oxidation
Rattus norvegicus
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
endoplasmic reticulum
-
Rattus norvegicus
5783
-
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
59000
-
SDS-PAGE
Rattus norvegicus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
albendazole + NADPH + O2
Rattus norvegicus
-
albendazole S-oxide + NADP+ + H2O
-
-
-
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
liver
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.013
-
-
Rattus norvegicus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
albendazole + NADPH + O2
-
285316
Rattus norvegicus
albendazole S-oxide + NADP+ + H2O
-
-
-
-
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
additional information
-
2 min. 50°C in assay buffer in the absence of NADPH inactivates the enzyme
Rattus norvegicus
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.4
-
assay conditions
Rattus norvegicus
Other publictions for EC 1.14.13.32
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
701177
Cvilink
Phase I biotransformation of a ...
Dicrocoelium dendriticum
Res. Vet. Sci.
86
49-55
2009
-
1
-
-
-
-
-
4
3
-
-
1
-
1
-
-
1
-
-
-
2
-
2
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-
-
-
-
-
-
2
-
-
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-
1
-
2
-
-
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-
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-
4
3
-
-
1
-
-
-
1
-
-
2
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
686548
Molina
Absorption and metabolism of a ...
Rattus norvegicus
Eur. J. Pharm. Sci.
31
16-24
2007
-
-
-
-
-
-
-
1
1
-
-
2
-
1
-
-
-
-
-
4
1
-
2
-
-
-
-
-
-
-
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2
-
-
-
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-
-
2
-
-
-
-
-
-
1
1
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2
-
-
-
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4
1
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
658526
Virkel
Comparative hepatic and extrah ...
Bos taurus, Ovis aries
Drug Metab. Dispos.
32
536-544
2004
-
-
-
-
-
-
-
-
2
-
-
-
-
2
-
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-
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2
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2
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-
-
-
-
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4
-
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4
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
2
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
285316
Moroni
Chiral sulfoxidation of albend ...
Rattus norvegicus
Drug Metab. Dispos.
23
160-165
1995
-
-
-
-
-
-
1
-
1
-
1
1
-
1
-
-
-
-
-
1
1
-
1
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
1
1
-
-
-
-
-
1
1
-
1
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
285315
Fargetton
Sulfoxidation of albendazole b ...
Rattus norvegicus, Sus scrofa
Vet. Res. Commun.
10
317-324
1986
-
-
-
-
-
-
2
1
2
-
-
2
-
3
-
-
-
-
-
2
1
-
2
-
-
-
-
-
1
-
-
4
-
-
-
-
-
-
4
-
-
-
-
2
-
1
2
-
-
2
-
-
-
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2
1
-
2
-
-
-
-
-
1
-
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