Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2,4-Dihydroxybenzoate | over 70% inhibition at 10 mM | Corynebacterium cyclohexanicum | |
2,5-Dihydroxybenzoate | over 70% inhibition at 10 mM | Corynebacterium cyclohexanicum | |
2-Hydroxybenzoate | over 70% inhibition at 10 mM | Corynebacterium cyclohexanicum | |
3-hydroxybenzoate | - |
Corynebacterium cyclohexanicum | |
4-Aminobenzoate | over 70% inhibition at 10 mM | Corynebacterium cyclohexanicum | |
4-Fluorobenzoate | - |
Corynebacterium cyclohexanicum | |
Cl- | non-competitive | Corynebacterium cyclohexanicum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.035 | - |
4-hydroxybenzoate | - |
Corynebacterium cyclohexanicum | |
0.045 | - |
NADH | pH 7.0-8.4 | Corynebacterium cyclohexanicum | |
0.063 | 0.17 | NADPH | Km increases as pH rises from 7.0 to 8.4 | Corynebacterium cyclohexanicum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
soluble | - |
Corynebacterium cyclohexanicum | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required for full activity in low concentrations of phosphate buffer, most effective at 5 mM | Corynebacterium cyclohexanicum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
47000 | - |
PAGE of native and denatured enzyme | Corynebacterium cyclohexanicum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Corynebacterium cyclohexanicum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Corynebacterium cyclohexanicum |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
30.5 | - |
NADPH | Corynebacterium cyclohexanicum |
Storage Stability | Organism |
---|---|
-20°C, several months | Corynebacterium cyclohexanicum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-hydroxybenzoate + NAD(P)H + O2 | enzyme is highly specific for 4-hydroxybenzoate, but uses NADH and NADPH at approximately equal rates | Corynebacterium cyclohexanicum | 3,4-dihydroxybenzoate + NAD(P)+ + H2O | - |
? | |
4-hydroxybenzoate + NADH + H+ + O2 | - |
Corynebacterium cyclohexanicum | 3,4-dihydroxybenzoate + NAD+ + H2O | - |
r |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 47000, PAGE of native and denatured enzyme | Corynebacterium cyclohexanicum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Corynebacterium cyclohexanicum |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
10 min, stable | Corynebacterium cyclohexanicum |
65 | - |
rapid inactivation above, p-hydroxybenzoate protects against heat inactivation | Corynebacterium cyclohexanicum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.1 | - |
NADPH | Corynebacterium cyclohexanicum |
7.8 | - |
NADH | Corynebacterium cyclohexanicum |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.2 | 8.8 | pH 6.2: about 50% of activity maximum, pH 8.8: about 70% of activity maximum, NADPH | Corynebacterium cyclohexanicum |
6.5 | 8.8 | 50% of activity maximum at pH 6.5 and pH 8.8, NADH | Corynebacterium cyclohexanicum |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4.3 | 8.5 | 4°C, 24 h, stable | Corynebacterium cyclohexanicum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | flavoprotein, 0.8 FAD per enzyme molecule | Corynebacterium cyclohexanicum | |
NADH | - |
Corynebacterium cyclohexanicum | |
NADPH | - |
Corynebacterium cyclohexanicum |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.029 | 0.045 | Cl- | - |
Corynebacterium cyclohexanicum |