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Literature summary for 1.14.13.33 extracted from

  • Kasai, D.; Fujinami, T.; Abe, T.; Mase, K.; Katayama, Y.; Fukuda, M.; Masai, E.
    Uncovering the protocatechuate 2,3-cleavage pathway genes (2009), J. Bacteriol., 191, 6758-6768.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
praI gene expressed in Escherichia coli BL21(DE3) cells harboring pETI23 Paenibacillus sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
41000
-
x * 41000, SDS-PAGE Paenibacillus sp.

Organism

Organism UniProt Comment Textmining
Paenibacillus sp.
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [Āµmol/min/mg] Specific Activity Maximum [Āµmol/min/mg] Comment Organism
0.36
-
for 4-hydroxybenzoate in the presence of NADPH Paenibacillus sp.
0.47
-
for 4-hydroxybenzoate in the presence of NADH Paenibacillus sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-hydroxybenzoate + NAD(P)H + O2
-
Paenibacillus sp. protocatechuate + NAD(P)+ + H2O
-
?

Subunits

Subunits Comment Organism
? x * 41000, SDS-PAGE Paenibacillus sp.

Synonyms

Synonyms Comment Organism
4HB 3-hydroxylase
-
Paenibacillus sp.

Cofactor

Cofactor Comment Organism Structure
NADH
-
Paenibacillus sp.
NADPH
-
Paenibacillus sp.