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Literature summary for 1.14.13.39 extracted from

  • Hevel, J.M.; Marletta, M.A.
    Macrophage nitric oxide synthase: relationship between enzyme-bound tetrahydrobiopterin and synthase activity (1992), Biochemistry, 31, 7160-7165.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
(6R,S)-Methyl-tetrahydropterin activation in the absence of biopterin, not as effective as tetrahydrobiopterin Mus musculus

General Stability

General Stability Organism
(6R)-tetrahydro-L-biopterin stabilizes during purification at 4°C Mus musculus
glycerol stabilizes Mus musculus

Inhibitors

Inhibitors Comment Organism Structure
6(R,S)-methyl-5-deazatetrahydropterin
-
Mus musculus
Trifluoperazine no inhibitor of macrophage enzyme Mus musculus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Mus musculus 5829
-

Organism

Organism UniProt Comment Textmining
Mus musculus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
2',5'-ADP-agarose affinity chromatography Mus musculus

Source Tissue

Source Tissue Comment Organism Textmining
macrophage
-
Mus musculus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.000073
-
purified enzyme, without tetrahydropterin Mus musculus
0.00035
-
purified enzyme, with tetrahydropterin Mus musculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 L-arginine + 3 NADPH + 4 O2 + 3 H+
-
Mus musculus 2 L-citrulline + 2 NO + 3 NADP+ + 4 H2O
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Mus musculus

Cofactor

Cofactor Comment Organism Structure
5,6,7,8-tetrahydro-L-biopterin enzyme purified in absence of biopterin contains substoichiometric concentration, if purified in presence of biopterin it contains 1 mol biopterin per mol MW 130000 subunit Mus musculus
5,6,7,8-tetrahydro-L-biopterin activity is correlated directly to bound biopterin concentration Mus musculus
FAD tightly enzyme-bound Mus musculus
FAD FAD containing flavoprotein Mus musculus
FMN tightly enzyme-bound Mus musculus
FMN FMN containing flavoprotein Mus musculus
NADPH
-
Mus musculus