Activating Compound | Comment | Organism | Structure |
---|---|---|---|
(6R,S)-Methyl-tetrahydropterin | activation in the absence of biopterin, not as effective as tetrahydrobiopterin | Mus musculus |
General Stability | Organism |
---|---|
(6R)-tetrahydro-L-biopterin stabilizes during purification at 4°C | Mus musculus |
glycerol stabilizes | Mus musculus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
6(R,S)-methyl-5-deazatetrahydropterin | - |
Mus musculus | |
Trifluoperazine | no inhibitor of macrophage enzyme | Mus musculus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Mus musculus | 5829 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
2',5'-ADP-agarose affinity chromatography | Mus musculus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
macrophage | - |
Mus musculus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.000073 | - |
purified enzyme, without tetrahydropterin | Mus musculus |
0.00035 | - |
purified enzyme, with tetrahydropterin | Mus musculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 L-arginine + 3 NADPH + 4 O2 + 3 H+ | - |
Mus musculus | 2 L-citrulline + 2 NO + 3 NADP+ + 4 H2O | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Mus musculus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
5,6,7,8-tetrahydro-L-biopterin | enzyme purified in absence of biopterin contains substoichiometric concentration, if purified in presence of biopterin it contains 1 mol biopterin per mol MW 130000 subunit | Mus musculus | |
5,6,7,8-tetrahydro-L-biopterin | activity is correlated directly to bound biopterin concentration | Mus musculus | |
FAD | tightly enzyme-bound | Mus musculus | |
FAD | FAD containing flavoprotein | Mus musculus | |
FMN | tightly enzyme-bound | Mus musculus | |
FMN | FMN containing flavoprotein | Mus musculus | |
NADPH | - |
Mus musculus |