Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.14.13.39 extracted from

  • Schmidt, H.H.H.W.; Smith, R.M.; Nakane, M.; Murad, F.
    Ca2+/calmodulin-dependent NO synthase type I: a biopteroflavoprotein with Ca2+/calmodulin-independent diaphorase and reductase activities (1992), Biochemistry, 31, 3243-3249.
    View publication on PubMed
Search for more literature for 1.14.13.39

General Stability

General Stability Organism
ammonium sulfate precipitation results in remarkable loss of tetrahydrobiopterin Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
2',3'-dialdehyde of NADPH at concentrations of 40times the apparent Km-value or after prolonged incubation, independent of Ca2+/calmodulin, L-arginine or tetrahydrobiopterin, NADPH prevents inhibition, the NADPH-diaphorase activity of the enzyme is less sensitive than the nitric oxide synthase activity Rattus norvegicus
additional information no inhibitor of NADPH-diaphorase activity: methotrexate Rattus norvegicus
nitroblue tetrazolium potent non-competitive inhibitor, partially reversible by tetrahydrobiopterin Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00002
-
 tetrahydrobiopterin
-
 Rattus norvegicus
0.0008
-
 2',3'-dialdehyde-NADPH
-
 Rattus norvegicus
0.002 0.0028 L-arginine NADPH Rattus norvegicus
0.0033 0.0043 L-arginine
-
 Rattus norvegicus
0.0323 0.035 L-arginine NADPH, diaphorase activity Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Rattus norvegicus 5829
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Rattus norvegicus NO represents the endogenous activator of soluble guanylyl cyclase ?
-
 ?
additional information Rattus norvegicus Sprague-Dawley NO represents the endogenous activator of soluble guanylyl cyclase ?
-
 ?

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
 Sprague-Dawley
-
Rattus norvegicus Sprague-Dawley
-
 Sprague-Dawley
-

Purification (Commentary)

Purification (Comment) Organism
-
 Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
 Rattus norvegicus
-
cerebellum
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.12
-
 NADPH-diaphorase activity Rattus norvegicus
0.17
-
-
 Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 L-arginine + 3 NADPH + 4 O2 + 3 H+
-
 Rattus norvegicus 2 L-citrulline + 2 NO + 3 NADP+ + 4 H2O the product is a guanylyl-cyclase-relaxing factor, that is identical with nitric oxide or a NO-releasing compound ?
2 L-arginine + 3 NADPH + 4 O2 + 3 H+
-
 Rattus norvegicus Sprague-Dawley 2 L-citrulline + 2 NO + 3 NADP+ + 4 H2O the product is a guanylyl-cyclase-relaxing factor, that is identical with nitric oxide or a NO-releasing compound ?
L-arginine + NADPH + O2 + tetrahydrobiopterin
-
 Rattus norvegicus citrulline + NO + NADP+ + ?
-
 ?
L-arginine + NADPH + O2 + tetrahydrobiopterin
-
 Rattus norvegicus Sprague-Dawley citrulline + NO + NADP+ + ?
-
 ?
additional information the enzyme exhibits NADPH-diaphorase activity, uncoupled from nitric oxide synthase activity Rattus norvegicus ?
-
 ?
additional information NO represents the endogenous activator of soluble guanylyl cyclase Rattus norvegicus ?
-
 ?
additional information the enzyme exhibits NADPH-diaphorase activity, uncoupled from nitric oxide synthase activity Rattus norvegicus Sprague-Dawley ?
-
 ?
additional information NO represents the endogenous activator of soluble guanylyl cyclase Rattus norvegicus Sprague-Dawley ?
-
 ?
nitroblue tetrazolium + 2 NADPH NADPH-diaphorase reaction Rattus norvegicus nitroblue tetrazolium formazan + 2 NADP+
-
 ?
nitroblue tetrazolium + 2 NADPH NADPH-diaphorase reaction Rattus norvegicus Sprague-Dawley nitroblue tetrazolium formazan + 2 NADP+
-
 ?
Nomega-hydroxy-L-arginine + 2',3'-dialdehyde-NADPH + H+ + O2
-
 Rattus norvegicus 2 L-citrulline + nitric oxide + 2',3'-dialdehyde-NADP+ + H2O
-
 r

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Rattus norvegicus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.65
-
 nitroblue tetrazolium NADPH-diaphorase activity Rattus norvegicus
1.08
-
 L-arginine
-
 Rattus norvegicus

Cofactor

Cofactor Comment Organism Structure
2',3'-dialdehyde analogue of NADPH activation, can substitute for NADPH at low concentrations, inhibitory at concentrations of 40times the apparent Km-value or after prolonged incubation Rattus norvegicus
5,6,7,8-tetrahydro-L-biopterin i.e. (6R)-2-amino-4-hydroxy-6-(L-erythro-1,2-dihydroxypropyl)-5,6,7,8-tetrahydropteridine, 6R-isomer, requirement, biopteroflavoprotein, 1 mol tetrahydrobiopterin per mol enzyme dimer Rattus norvegicus
Calmodulin NADPH-diaphorase activity of the enzyme is Ca2+/calmodulin independent Rattus norvegicus
FAD 1 mol FAD per mol enzyme dimer Rattus norvegicus
FMN 1 mol FMN per mol enzyme dimer Rattus norvegicus
NADPH requirement, specific for, NADPH-diaphorase activity requires higher NADPH concentrations than nitric oxide formation Rattus norvegicus