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Literature summary for 1.14.13.39 extracted from
- Purification of insoluble nitric oxide synthase from rat cerebellum (1992), J. Biochem., 111, 556-558.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.014 | 0.018 | L-arginine | - |
Rattus norvegicus |  |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Rattus norvegicus | 5829 | - |
| membrane | - |
Rattus norvegicus | 16020 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | cerebellum enzyme is Ca2+-dependent | Rattus norvegicus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 150000 | - |
1 * 150000, SDS-PAGE | Rattus norvegicus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
Wistar strain | - |
| Rattus norvegicus | - |
2 isoforms | - |
| Rattus norvegicus Wistar | - |
Wistar strain | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| sequential affinity chromatography on 2',5'-ADP-agarose and calmodulin Sepharose 4B | Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| brain | Ca2+-dependent isoform | Rattus norvegicus | - |
| cerebellum | - |
Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.0098 | - |
purified enzyme | Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2 L-arginine + 3 NADPH + 4 O2 + 3 H+ | - |
Rattus norvegicus | 2 L-citrulline + 2 NO + 3 NADP+ + 4 H2O | - |
? | |
| 2 L-arginine + 3 NADPH + 4 O2 + 3 H+ | - |
Rattus norvegicus Wistar | 2 L-citrulline + 2 NO + 3 NADP+ + 4 H2O | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 150000, SDS-PAGE | Rattus norvegicus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Rattus norvegicus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| Calmodulin | - |
Rattus norvegicus | |
| FAD | absolute requirement for FAD | Rattus norvegicus | |
| NADPH | dependent on | Rattus norvegicus | |
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Release 2023.1 (January 2023)
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