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Literature summary for 1.14.13.39 extracted from

  • Yui, Y.; Hattori, R.; Kosuga, K.; Eizawa, H.;Hiki, K.; Kawai, C.
    Purification of nitric oxide synthase from rat macrophages (1991), J. Biol. Chem., 266, 12544-12547.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
dithiothreitol requirement Rattus norvegicus

General Stability

General Stability Organism
dithiothreitol stabilizes Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
ethylene glycol bis(beta-amino-ethylether)-N,N,N',N'-tetraacetic acid i.e. EGTA, complete inhibition of cytosolic enzyme, partial inhibition of particulate enzyme Rattus norvegicus
additional information the macrophage enzyme is not inhibited by calmodulin antagonists (N-4-aminobutyl-), (N-6-aminohexyl)-5-chloro-2-naphthalene sulfonamide; the macrophage enzyme is not inhibited by calmodulin antagonists (N-6-aminohexyl)-1-naphthalene sulfonamide Rattus norvegicus
Trifluoperazine no inhibitor of macrophage enzyme Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0323 0.035 L-arginine
-
Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
150000
-
2 * 150000, SDS-PAGE Rattus norvegicus
300000
-
gel filtration Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
macrophage
-
Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.94 0.96 purified enzyme Rattus norvegicus

Storage Stability

Storage Stability Organism
4°C, crude, stable for at least 24 h Rattus norvegicus
4°C, purified, t1/2: 6 h at pH 7.4 Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 L-arginine + 3 NADPH + 4 O2 + 3 H+
-
Rattus norvegicus 2 L-citrulline + 2 NO + 3 NADP+ + 4 H2O
-
?

Subunits

Subunits Comment Organism
dimer 2 * 150000, SDS-PAGE Rattus norvegicus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 8
-
Rattus norvegicus

pH Stability

pH Stability pH Stability Maximum Comment Organism
7.4
-
purified, t1/2: 6 h, 4°C Rattus norvegicus

Cofactor

Cofactor Comment Organism Structure
5,6,7,8-tetrahydro-L-biopterin required Rattus norvegicus
FAD
-
Rattus norvegicus
NADPH dependent on Rattus norvegicus