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Literature summary for 1.14.13.39 extracted from
- Kinetic characteristics of nitric oxide synthase from rat brain (1990), Biochem. J., 269, 207-210.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ngamma-iminoethyl-L-ornithine | competitive inhibitor | Rattus norvegicus |  |
| Ngamma-monomethyl-L-arginine | not D-isomer, strong, competitive | Rattus norvegicus | |
| Ngamma-nitro-L-arginine | competitive inhibitor | Rattus norvegicus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.008 | 0.0084 | dichlorophenolindophenol | with L-arginine | Rattus norvegicus | |
| 0.0084 | - |
L-arginine | - |
Rattus norvegicus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Rattus norvegicus | 5829 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | - |
Rattus norvegicus | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Rattus norvegicus | NO represents the endogenous activator of soluble guanylyl cyclase | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| brain | - |
Rattus norvegicus | - |
| forebrain | synaptosomal fraction | Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.000043 | - |
crude extract | Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2 L-arginine + 3 NADPH + 4 O2 + 3 H+ | - |
Rattus norvegicus | 2 L-citrulline + 2 NO + 3 NADP+ + 4 H2O | the product is a guanylyl-cyclase-relaxing factor, that is identical with nitric oxide or a NO-releasing compound | ? | |
| 2,6-dichlorophenolindophenol + NADPH + O2 | - |
Rattus norvegicus | ? + NO + NADP+ | - |
? | |
| additional information | NO represents the endogenous activator of soluble guanylyl cyclase | Rattus norvegicus | ? | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Rattus norvegicus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.2 | - |
assay at | Rattus norvegicus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADPH | dependent on | Rattus norvegicus | |
| NADPH | crude preparation requires only NADPH as cofactor | Rattus norvegicus | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0004 | - |
Ngamma-nitro-L-arginine | - |
Rattus norvegicus | |
| 0.0007 | - |
Ngamma-monomethyl-L-arginine | - |
Rattus norvegicus | |
| 0.0012 | - |
Ngamma-iminoethyl-L-ornithine | - |
Rattus norvegicus | |
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Release 2023.1 (January 2023)
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