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Literature summary for 1.14.13.39 extracted from

  • Iwasaki, T.; Hori, H.; Hayashi, Y.; Nishino, T.; Tamura, K.; Oue, S.; Iizuka, T.; Ogura, T.; Esumi, H.
    Characterization of mouse nNOS2, a natural variant of neuronal nitric-oxide synthase produced in the central nervous system by selective alternative splicing (1999), J. Biol. Chem., 274, 17559-17566.
    View publication on PubMed
Search for more literature for 1.14.13.39

Cloned(Commentary)

Cloned (Comment) Organism
neuronal wild-type and natural mutant, specifically expressed in the central nervous system, expression in Escherichia coli and in insect cells of Spodoptera frugiperda via baculovirus infection Mus musculus

Crystallization (Commentary)

Crystallization (Comment) Organism
x-ray crystal structure of the heme-binding domains of neuronal wild-type and mutant with deletion in the heme-binding domain Mus musculus

Protein Variants

Protein Variants Comment Organism
additional information naturally occuring neuronal mutant with a 105-amino acid deletion in the heme-binding domain as a result of in-frame mutation by specific alternative splicing, contains heme, but shows no L-arginine and NADPH-dependent citrulline-forming activity in presence of Ca2+-promoted calmodulin Mus musculus

Metals/Ions

Metals/Ions Comment Organism Structure
Iron naturally occuring neuronal mutant with a 105-amino acid deletion in the heme-binding domain as a result of in-frame mutation by specific alternative splicing, contains heme, but shows no L-arginine and NADPH-dependent citrulline-forming activity in presence of Ca2+-promoted calmodulin, the heme coordination geometry is highly abnormal Mus musculus

Organism

Organism UniProt Comment Textmining
Mus musculus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
wild-type and natural mutant, recombinant from Escherichia coli and insect cells Mus musculus

Reaction

Reaction Comment Organism Reaction ID
2 L-arginine + 3 NADPH + 3 H+ + 4 O2 = 2 L-citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O alignment of heme-binding domain amino acid sequences of NOS Mus musculus
a

Source Tissue

Source Tissue Comment Organism Textmining
neuron central nervous system, natural variant with 105-amino acid deletion in the heme-binding domain Mus musculus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 L-arginine + 3 NADPH + 4 O2 + 3 H+
-
 Mus musculus 2 L-citrulline + 2 NO + 3 NADP+ + 4 H2O
-
 ?

Cofactor

Cofactor Comment Organism Structure
NADPH
-
 Mus musculus