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Literature summary for 1.14.13.39 extracted from

  • Conrad, K.P.; Powers, R.W.; Davis, A.K.; Novak, J.
    Citrulline is not the major product using the standard NOS activity assay on renal cortical homogenates (2002), Am. J. Physiol., 282, R303-R310.
    View publication on PubMed
Search for more literature for 1.14.13.39

Inhibitors

Inhibitors Comment Organism Structure
Ngamma-monomethyl-L-arginine slightly Rattus norvegicus
Ngamma-nitro-L-arginine methyl ester very slightly, only L-isomer and in presence of tetrahydrobiopterin and NADPH Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.097
-
 L-arginine with Ca2+, 0.01 mM Rattus norvegicus
0.11
-
 L-arginine without Ca2+ Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Rattus norvegicus 5829
-
membrane
-
 Rattus norvegicus 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ required Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
 Sprague-Dawley
-
Rattus norvegicus Sprague-Dawley
-
 Sprague-Dawley
-

Source Tissue

Source Tissue Comment Organism Textmining
kidney renal cortex Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.000000305
-
 crude extract Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 L-arginine + 3 NADPH + 4 O2 + 3 H+
-
 Rattus norvegicus 2 L-citrulline + 2 NO + 3 NADP+ + 4 H2O
-
 ?
2 L-arginine + 3 NADPH + 4 O2 + 3 H+
-
 Rattus norvegicus Sprague-Dawley 2 L-citrulline + 2 NO + 3 NADP+ + 4 H2O
-
 ?

Cofactor

Cofactor Comment Organism Structure
5,6,7,8-tetrahydro-L-biopterin
-
 Rattus norvegicus
Calmodulin
-
 Rattus norvegicus
FAD
-
 Rattus norvegicus
NADPH
-
 Rattus norvegicus