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Literature summary for 1.14.13.39 extracted from

  • Demady, D.R.; Jianmongkol, S.; Vuletich, J.L.; Bender, A.T.; Osawa, Y.
    Agmatine enhances the NADPH oxidase activity of neuronal NO synthase and leads to oxidative inactivation of the enzyme (2001), Mol. Pharmacol., 59, 24-29.
    View publication on PubMed
Search for more literature for 1.14.13.39

Application

Application Comment Organism
pharmacology NO synthase can be used to gain insight into the biological role of endogenous agmatine Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
neuronal enzyme expressed in Spodoptera frugiperda cells via baculovirus infection Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
agmatine at lower concentration than the Ki value agmatine leads to time-, concentration-, NADPH- and calmodulin-dependent inhibition of the neuronal enzyme in presence of calmodulin; causes an increase in NADPH oxidase activity of the enzyme Homo sapiens
H2O2 alters heme group, decrease in activity Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Homo sapiens 5829
-

Metals/Ions

Metals/Ions Comment Organism Structure
Iron 0.88 mol per mol of recombinant enzyme monomer Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
wild-type recombinant from insect cells Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
neuron
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 assay method Homo sapiens
1
-
 purified enzyme Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 L-arginine + 3 NADPH + 4 O2 + 3 H+
-
 Homo sapiens 2 L-citrulline + 2 NO + 3 NADP+ + 4 H2O
-
 ?

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.66
-
 agmatine at lower concentration than the Ki value agmatine leads to time-, concentration-, NADPH- and calmodulin-dependent inhibition of the neuronal enzyme in presence of calmodulin Homo sapiens