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Literature summary for 1.14.13.39 extracted from

  • Wolff, D.J.; Lubeskie, A.; Umansky, S.
    The inhibition of the constitutive bovine endothelial nitric oxide synthase by imidazole and indazole agents (1994), Arch. Biochem. Biophys., 314, 360-366.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
dithiothreitol
-
Bos taurus

Inhibitors

Inhibitors Comment Organism Structure
1-phenylimidazole reversible inhibition of endothelial enzyme, competitive versus L-arginine and tetrahydrobiopterin, no inhibition of cytochrome c reduction Bos taurus
7-nitroindazole reversible inhibition of endothelial enzyme, competitive versus tetrahydrobiopterin, no inhibition of cytochrome c reduction Bos taurus
imidazole inhibition of the endothelial enzyme, competitive versus L-arginine, no inhibition of cytochrome c reduction Bos taurus

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ required Bos taurus

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
endothelium constitutive, from pulmonary artery Bos taurus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 L-arginine + 3 NADPH + 4 O2 + 3 H+
-
Bos taurus 2 L-citrulline + 2 NO + 3 NADP+ + 4 H2O
-
?

Cofactor

Cofactor Comment Organism Structure
5,6,7,8-tetrahydro-L-biopterin stimulates Bos taurus
Calmodulin dependent on, endothelial enzyme Bos taurus
NADPH
-
Bos taurus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0008
-
7-nitroindazole
-
Bos taurus
0.05
-
imidazole
-
Bos taurus
0.05
-
1-phenylimidazole
-
Bos taurus