Literature summary for 1.14.13.39 extracted from

Gerber, N.C.; Nishida, C.R.; de Montellano, P.R.O.
Characterization of human liver inducible nitric oxide synthase expressed in Escherichia coli (1997), Arch. Biochem. Biophys., 343, 249-253.

Search for more literature for 1.14.13.39

Cloned(Commentary)

Cloned (Comment)	Organism
inducible liver isoform is expressed in Escherichia coli with and without His-tag, requires coexpression of calmodulin	Homo sapiens

General Stability

General Stability	Organism
inducible isoform is unstable during purification in absence of L-arginine and tetrahydrobiopterin toward loss of the heme group and formation of low-spin species	Homo sapiens

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.022	-	L-arginine	in presence of tetrahydrobiopterin and L-arginine	Homo sapiens

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Ca2+	no stimulation with exogenous Ca2+, inducible isoform from liver	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	inducible	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant wild-type of inducible liver isoform from Escherichia coli with and without His-tag, requires inclusion of tetrahydrobiopterin in purification buffer	Homo sapiens

Source Tissue

Source Tissue	Comment	Organism	Textmining
liver	inducible isoform	Homo sapiens	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
38	-	purified enzyme, inducible isoform, cytochrome c reductase activity	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2 L-arginine + 3 NADPH + 4 O2 + 3 H+	-	Homo sapiens	2 L-citrulline + 2 NO + 3 NADP+ + 4 H2O	-	?

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	assay at	Homo sapiens

Cofactor

Cofactor	Comment	Organism	Structure
Calmodulin	no stimulation with exogenous calmodulin, inducible isoform from liver	Homo sapiens
FAD	no flavin	Homo sapiens
FMN	no flavin	Homo sapiens

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Release 2023.1 (January 2023)