KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | rapid kinetic analysis, stopped-flow measurements under high pressure | Homo sapiens | |
additional information | - |
additional information | rapid kinetic analysis, stopped-flow measurements under high pressure | Rattus norvegicus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | - |
Homo sapiens | |
Fe2+ | - |
Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 L-arginine + 3 NADPH + 4 O2 + 3 H+ | Homo sapiens | - |
2 L-citrulline + 2 NO + 3 NADP+ + 4 H2O | - |
? | |
2 L-arginine + 3 NADPH + 4 O2 + 3 H+ | Rattus norvegicus | - |
2 L-citrulline + 2 NO + 3 NADP+ + 4 H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Rattus norvegicus | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
2 L-arginine + 3 NADPH + 3 H+ + 4 O2 = 2 L-citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O | reaction mechanism, the reaction of the enzyme with oxygen is fast and takes place within several steps, separated by ephemeral intermediates, two short-lived oxy-compounds OxyI and OxyII | Homo sapiens | |
2 L-arginine + 3 NADPH + 3 H+ + 4 O2 = 2 L-citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O | reaction mechanism, the reaction of the enzyme with oxygen is fast and takes place within several steps, separated by ephemeral intermediates, two short-lived oxy-compounds OxyI and OxyII | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | neuronal enzyme | Homo sapiens | - |
brain | neuronal enzyme | Rattus norvegicus | - |
endothelium | endothelial enzyme | Homo sapiens | - |
endothelium | endothelial enzyme | Rattus norvegicus | - |
macrophage | inducible enzyme | Homo sapiens | - |
macrophage | inducible enzyme | Rattus norvegicus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 L-arginine + 3 NADPH + 4 O2 + 3 H+ | - |
Homo sapiens | 2 L-citrulline + 2 NO + 3 NADP+ + 4 H2O | - |
? | |
2 L-arginine + 3 NADPH + 4 O2 + 3 H+ | - |
Rattus norvegicus | 2 L-citrulline + 2 NO + 3 NADP+ + 4 H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
endothelial nitric oxide synthase | - |
Homo sapiens |
endothelial nitric oxide synthase | - |
Rattus norvegicus |
eNOS | - |
Homo sapiens |
eNOS | - |
Rattus norvegicus |
inducible nitric oxide synthase | - |
Homo sapiens |
inducible nitric oxide synthase | - |
Rattus norvegicus |
iNOS | - |
Homo sapiens |
iNOS | - |
Rattus norvegicus |
neuronal nitric oxide synthase | - |
Homo sapiens |
neuronal nitric oxide synthase | - |
Rattus norvegicus |
nNOS | - |
Homo sapiens |
nNOS | - |
Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Homo sapiens | |
NADPH | - |
Rattus norvegicus | |
tetrahydrobiopterin | oxidation product of BH4 is a protonated BH3 radical, key role of BH4 in protonation of Fe(II)-O2-, overview | Homo sapiens | |
tetrahydrobiopterin | oxidation product of BH4 is a protonated BH3 radical, key role of BH4 in protonation of Fe(II)-O2-, overview | Rattus norvegicus |