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Literature summary for 1.14.13.39 extracted from
- Hunting oxygen complexes of nitric oxide synthase at low temperature and high pressure (2005), Biochem. Biophys. Res. Commun., 338, 529-535.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | rapid kinetic analysis, stopped-flow measurements under high pressure | Homo sapiens | |
| additional information | - |
additional information | rapid kinetic analysis, stopped-flow measurements under high pressure | Rattus norvegicus |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Fe2+ | - |
Homo sapiens |  |
| Fe2+ | - |
Rattus norvegicus | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2 L-arginine + 3 NADPH + 4 O2 + 3 H+ | Homo sapiens | - |
2 L-citrulline + 2 NO + 3 NADP+ + 4 H2O | - |
? | |
| 2 L-arginine + 3 NADPH + 4 O2 + 3 H+ | Rattus norvegicus | - |
2 L-citrulline + 2 NO + 3 NADP+ + 4 H2O | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
| Rattus norvegicus | - |
- |
- |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| 2 L-arginine + 3 NADPH + 3 H+ + 4 O2 = 2 L-citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O | reaction mechanism, the reaction of the enzyme with oxygen is fast and takes place within several steps, separated by ephemeral intermediates, two short-lived oxy-compounds OxyI and OxyII | Homo sapiens | |
| 2 L-arginine + 3 NADPH + 3 H+ + 4 O2 = 2 L-citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O | reaction mechanism, the reaction of the enzyme with oxygen is fast and takes place within several steps, separated by ephemeral intermediates, two short-lived oxy-compounds OxyI and OxyII | Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| brain | neuronal enzyme | Homo sapiens | - |
| brain | neuronal enzyme | Rattus norvegicus | - |
| endothelium | endothelial enzyme | Homo sapiens | - |
| endothelium | endothelial enzyme | Rattus norvegicus | - |
| macrophage | inducible enzyme | Homo sapiens | - |
| macrophage | inducible enzyme | Rattus norvegicus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2 L-arginine + 3 NADPH + 4 O2 + 3 H+ | - |
Homo sapiens | 2 L-citrulline + 2 NO + 3 NADP+ + 4 H2O | - |
? | |
| 2 L-arginine + 3 NADPH + 4 O2 + 3 H+ | - |
Rattus norvegicus | 2 L-citrulline + 2 NO + 3 NADP+ + 4 H2O | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| endothelial nitric oxide synthase | - |
Homo sapiens |
| endothelial nitric oxide synthase | - |
Rattus norvegicus |
| eNOS | - |
Homo sapiens |
| eNOS | - |
Rattus norvegicus |
| inducible nitric oxide synthase | - |
Homo sapiens |
| inducible nitric oxide synthase | - |
Rattus norvegicus |
| iNOS | - |
Homo sapiens |
| iNOS | - |
Rattus norvegicus |
| neuronal nitric oxide synthase | - |
Homo sapiens |
| neuronal nitric oxide synthase | - |
Rattus norvegicus |
| nNOS | - |
Homo sapiens |
| nNOS | - |
Rattus norvegicus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADPH | - |
Homo sapiens | |
| NADPH | - |
Rattus norvegicus | |
| tetrahydrobiopterin | oxidation product of BH4 is a protonated BH3 radical, key role of BH4 in protonation of Fe(II)-O2-, overview | Homo sapiens | |
| tetrahydrobiopterin | oxidation product of BH4 is a protonated BH3 radical, key role of BH4 in protonation of Fe(II)-O2-, overview | Rattus norvegicus | |
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Release 2023.1 (January 2023)
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