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Literature summary for 1.14.13.39 extracted from

  • Ilagan, R.P.; Tiso, M.; Konas, D.W.; Hemann, C.; Durra, D.; Hille, R.; Stuehr, D.J.
    Differences in a conformational equilibrium distinguish catalysis by the endothelial and neuronal nitric-oxide synthase flavoproteins (2008), J. Biol. Chem., 283, 19603-19615.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.14.13.39

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information kinetics of cytcochrome c reduction and of reaction with FMN and cytochrome c, overview, eNOS and nNOS differ slightly in their NADP(H) interaction and flavin thermodynamics, and show distinct electron transfer activities, two-state conformational equilibrium of the FMN subdomain, overview Bos taurus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2 L-arginine + 3 NADPH + 3 H+ + 4 O2 Bos taurus
-
 2 citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Bos taurus
-
 isozymes eNOS and nNOS
-

Source Tissue

Source Tissue Comment Organism Textmining
endothelium
-
 Bos taurus
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neuron
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 low eNOS activity might involve an altered interaction with NADP(H) Bos taurus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 L-arginine + 3 NADPH + 3 H+ + 4 O2
-
 Bos taurus 2 citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O
-
 ?
2 L-arginine + 3 NADPH + 3 H+ + 4 O2 the electron transfer between cofactors FMN and FAD is reversible Bos taurus 2 citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O
-
 ?
additional information endothelial NOS has a 6fold lower NO synthesis activity and 6-16fold lower cytochrome c reductase activity than neuronal NOS due to a significantly different electron transfer capacities, substrate specificity and mechanism, oveview Bos taurus ?
-
 ?

Synonyms

Synonyms Comment Organism
endothelial nitric-oxide synthase
-
 Bos taurus
endothelial NOS
-
 Bos taurus
eNOS
-
 Bos taurus
neuronal nitric-oxide synthase
-
 Bos taurus
neuronal NOS
-
 Bos taurus
nNOS
-
 Bos taurus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
10 25 assay at Bos taurus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.6
-
 assay at Bos taurus

Cofactor

Cofactor Comment Organism Structure
cytochrome c
-
 Bos taurus
FAD
-
 Bos taurus
FMN
-
 Bos taurus
NADP+ binding mechanism Bos taurus
NADPH binding mechanism Bos taurus