Cloned (Comment) | Organism |
---|---|
the wild type and the DELTAG810 mutant of nNOS are cloned in Escherichia coli strain DH5alpha | Rattus norvegicus |
Protein Variants | Comment | Organism |
---|---|---|
additional information | the DELTAG810 mutant enzyme shows strongly reduced activity towards nitric oxide synthesis and NADPH oxidation compared to the wild type enzyme | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | P29476 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 L-arginine + 3 NADPH + 3 H+ + 4 O2 | - |
Rattus norvegicus | 2 citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
neuronal nitric-oxide synthase | - |
Rattus norvegicus |
nNOS | - |
Rattus norvegicus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.033 | - |
nitric oxide | DELTAG810 mutant enzyme, in 25 mM potassium phosphate, pH 7.5, with 100 mM KCl, at 25°C | Rattus norvegicus | |
0.055 | - |
nitric oxide | DELTAG810 mutant enzyme, in the presence of 10 units/ml catalase and superoxide dismutase, in 25 mM potassium phosphate, pH 7.5, with 100 mM KCl, at 25°C | Rattus norvegicus | |
0.28 | - |
NADPH | DELTAG810 mutant enzyme, in the presence of 10 units/ml catalase and superoxide dismutase, in 25 mM potassium phosphate, pH 7.5, with 100 mM KCl, at 25°C | Rattus norvegicus | |
0.32 | - |
NADPH | DELTAG810 mutant enzyme, in 25 mM potassium phosphate, pH 7.5, with 100 mM KCl, at 25°C | Rattus norvegicus | |
0.92 | - |
nitric oxide | wild type enzyme, in 25 mM potassium phosphate, pH 7.5, with 100 mM KCl, at 25°C | Rattus norvegicus | |
1.23 | - |
nitric oxide | wild type enzyme, in the presence of 10 units/ml catalase and superoxide dismutase, in 25 mM potassium phosphate, pH 7.5, with 100 mM KCl, at 25°C | Rattus norvegicus | |
2.33 | - |
NADPH | wild type enzyme, in the presence of 10 units/ml catalase and superoxide dismutase, in 25 mM potassium phosphate, pH 7.5, with 100 mM KCl, at 25°C | Rattus norvegicus | |
2.57 | - |
NADPH | wild type enzyme, in 25 mM potassium phosphate, pH 7.5, with 100 mM KCl, at 25°C | Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
5,6,7,8-tetrahydro-L-biopterin | - |
Rattus norvegicus | |
Calmodulin | - |
Rattus norvegicus | |
NADPH | - |
Rattus norvegicus |