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Literature summary for 1.14.13.39 extracted from

  • Di Natale, M.R.; Hunne, B.; Liew, J.J.M.; Fothergill, L.J.; Stebbing, M.J.; Furness, J.B.
    Morphologies, dimensions and targets of gastric nitric oxide synthase neurons (2022), Cell Tissue Res., 388, 19-32 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.14.13.39

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-arginine + 3 NADPH + 3 H+ + 4 O2 Rattus norvegicus overall reaction 2 L-citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O
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 ?
L-arginine + 3 NADPH + 3 H+ + 4 O2 Rattus norvegicus Sprague-Dawley overall reaction 2 L-citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O
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 ?

Organism

Organism UniProt Comment Textmining
Rattus norvegicus P29476
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Rattus norvegicus Sprague-Dawley P29476
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Source Tissue

Source Tissue Comment Organism Textmining
additional information immunohistochemic enzyme and NADPH histochemic analysis, distributions of nNOS neurons and their terminals throughout the rat stomach, overview. NADPH diaphorase is the colour reaction that reveals the presence of NADPH oxidases, and because nNOS is a NADPH oxidase, it is revealed by NADPHd histochemistry Rattus norvegicus
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neuron nitrergic neurons in the rat stomach, the myenteric cell bodies have single axons, type I morphology and a wide range of sizes. Nitrergic terminals do not provide baskets of terminals around myenteric neurons. The nitrergic neuron populations in the stomach supply the muscle layers and intramural arteries, but, unlike in the intestine, gastric interneurons do not express nNOS Rattus norvegicus
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stomach nitrergic neurons in the rat stomach comprising similar proportions of myenteric neurons, about 30%, in all gastric regions, e.g. the longitudinal, circular and oblique layers of the external muscle, the muscularis mucosae and arteries within the gastric wall Rattus norvegicus
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-arginine + 3 NADPH + 3 H+ + 4 O2 overall reaction Rattus norvegicus 2 L-citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O
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 ?
L-arginine + 3 NADPH + 3 H+ + 4 O2 overall reaction Rattus norvegicus Sprague-Dawley 2 L-citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O
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 ?

Synonyms

Synonyms Comment Organism
NADPH diaphorase
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 Rattus norvegicus
NADPHd
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 Rattus norvegicus
neuronal nitric oxide synthase
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 Rattus norvegicus
nitric oxide synthase
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 Rattus norvegicus
nNOS
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 Rattus norvegicus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
22
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 assay at room temperature Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2
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 assay at Rattus norvegicus

Cofactor

Cofactor Comment Organism Structure
NADPH
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 Rattus norvegicus

General Information

General Information Comment Organism
additional information the large numbers of nNOS neurons and the density of innervation of the circular muscle and pyloric sphincter suggest that there is a finely graded control of motor function in the stomach by the recruitment of different numbers of inhibitory motor neurons Rattus norvegicus
physiological function nitrergic neurons in the rat stomach supply the longitudinal, circular and oblique layers of the external muscle, the muscularis mucosae and arteries within the astric wall. A small number of fibres are in the mucosa. All the neurons appear to have a single axon and in most cases a type I morphology. Type I is the typical morphology of enteric motor neurons that supply the muscle of the gastrointestinal tract. Neuronal nitric oxide synthase (nNOS) neurons do not supply terminals around myenteric nerve cells, whether NADPHd histochemistry or nNOS immunohistochemistry is used to locate the terminals of nNOS neurons. Thus, nNOS appears to be exclusively in motor neurons supplying the muscle of the rat stomach. This may be different from other species. nNOS immunoreactivity identifies 4 classes of motor neurons that supply the muscle of the rat stomach, an analysis of cell body sizes does not identify separate grouping of neurons. The rat stomach harbours large numbers of nNOS neurons, all or almost all of which are motor neurons supplying the external muscle, the muscularis mucosae and intramural arteries Rattus norvegicus