Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-hydroxybiphenyl + NADH + H+ + O2 | Pseudomonas nitroreducens | - |
2,3-dihydroxybiphenyl + NAD+ + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas nitroreducens | O06647 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-hydroxybiphenyl + NADH + H+ + O2 | - |
Pseudomonas nitroreducens | 2,3-dihydroxybiphenyl + NAD+ + H2O | - |
? | |
additional information | comparisons of substrate binding structures, overview | Pseudomonas nitroreducens | ? | - |
- |
Synonyms | Comment | Organism |
---|---|---|
2-hydroxybiphenyl 3-monooxygenase | - |
Pseudomonas nitroreducens |
HbpA | - |
Pseudomonas nitroreducens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | binding domain structure comparisons, overview | Pseudomonas nitroreducens | |
NADH | - |
Pseudomonas nitroreducens |
General Information | Comment | Organism |
---|---|---|
additional information | sequence comparisons, three-dimensional enzyme structure analysis, and structure comparisons with 2-hydroxybiphenyl 3-monooxygenase (HbpA, PDB ID 4cy8) from Pseudomonas nitroreducens and 2-methyl-3-hydroxypyridine-5-carboxylic acid oxygenase (MHPCO) from Mesorhizobium japonicum, overview. Despite having only 14% similarity in their primary sequences, pairwise structure alignments of PobA from Pseudomonas putida with HbpA from Pseudomonas nitroreducens and MHPCO from Mesorhizobium japonicum reveal local similarities between these structures. Key residues in the FAD-binding and substrate-binding sites of PobA are highly conserved spatially across the proteins from all three species. Key secondary-structure elements important for catalysis, such as the betaalphabeta fold, beta-sheet wall and alpha12 helix, are conserved across this expanded class of oxygenases | Pseudomonas nitroreducens |
physiological function | HbpA hydroxylates its substrate 2-hydroxybiphenyl to 2,3-dihydroxybiphenyl | Pseudomonas nitroreducens |