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Literature summary for 1.14.13.5 extracted from

  • Maki, Y.; Yamamoto, S.; Nozaki, M.; Hayaishi, O.
    Studies on monooxygenases. II. Crystallization and some properties of imidazole acetate monooxygenase (1969), J. Biol. Chem., 244, 2942-2950.
    View publication on PubMed

Crystallization (Commentary)

Crystallization (Comment) Organism
-
Pseudomonas sp.

Inhibitors

Inhibitors Comment Organism Structure
indoleacetate 30% inhibition at 10 mM Pseudomonas sp.
mercury compounds
-
Pseudomonas sp.
nicotinate 65% inhibition at 20 mM Pseudomonas sp.
p-chloromercuribenzoate
-
Pseudomonas sp.
phenylacetate 79% inhibition at 20 mM Pseudomonas sp.
silver compounds
-
Pseudomonas sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.002 0.01 NADH
-
Pseudomonas sp.
0.01
-
NADH spectrophotometric method Pseudomonas sp.
0.01
-
Sodium diethyldithiocarbamate polarographic method Pseudomonas sp.
0.015
-
NADH substrate: sodium diethyldithiocarbamate, polarographic method Pseudomonas sp.
0.02
-
O2 substrate: imidazoleacetate or sodium diethyldithiocarbamate, polarographic method Pseudomonas sp.
0.03
-
NADH polarographic method Pseudomonas sp.
0.3
-
imidazoleacetate spectrophotometric method Pseudomonas sp.
0.5
-
imidazoleacetate polarographic method Pseudomonas sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
87000 90000 sedimentation equilibrium, gel filtration Pseudomonas sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4-imidazoleacetate + NADH + O2 Pseudomonas sp.
-
5-hydroxy-4-imidazoleacetate + NAD+ + H2O
-
?

Organism

Organism UniProt Comment Textmining
Pseudomonas sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
using protamine sulfate treatment, ammonium sulfate fractionation, chromatography on TEAE-cellulose column, calcium phosphate gel treatment and chromatography on DEAE-Sephadex column Pseudomonas sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
25
-
-
Pseudomonas sp.

Storage Stability

Storage Stability Organism
-20°C, potassium phosphate buffer, pH 7.2, no loss of activity for at least 1 month Pseudomonas sp.
4°C, 5-100 mM potassium phosphate buffer, pH 7.2, almost full activity retained for several days Pseudomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-imidazoleacetate + NAD(P)H + O2 + H+ electrons can be trasferred from NADH to 2,6-dichloroindophenol in the presence of the enzyme Pseudomonas sp. 5-hydroxy-4-imidazoleacetate + NAD(P)+ + H2O
-
?
4-imidazoleacetate + NADH + O2
-
Pseudomonas sp. 5-hydroxy-4-imidazoleacetate + NAD+ + H2O
-
?
imidazolelactate + NADH + O2 + H+
-
Pseudomonas sp. 5-hydroxyimidazolelactate + NAD+ + H2O
-
?
imidazolepropionate + NAD(P)H + O2 + H+
-
Pseudomonas sp. 5-hydroxyimidazolepropionate + NAD(P)+ + H2O
-
?
sodium diethyldithiocarbamate + NADH + O2
-
Pseudomonas sp. ? + NAD+ + H2O reaction product not identified ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
20
-
2% loss of activity, 0.1 M potassium phosphate, pH 7.2, 5 min Pseudomonas sp.
30
-
50% loss of activity, 0.1 M potassium phosphate, pH 7.2, 5 min Pseudomonas sp.
40
-
95% loss of activity, 0.1 M potassium phosphate, pH 7.2, 5 min Pseudomonas sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
37.7
-
imidazoleacetate
-
Pseudomonas sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
-
Pseudomonas sp.

Cofactor

Cofactor Comment Organism Structure
FAD 1 mol per mol enzyme Pseudomonas sp.
FAD cannot be replaced by FMN or riboflavin Pseudomonas sp.
NADH
-
Pseudomonas sp.
NADPH beta-NADPH is approximately 13% as active as beta-NADH Pseudomonas sp.
NADPH alpha-NADH cannot replace beta-NADH at 0.2 mM Pseudomonas sp.