BRENDA - Enzyme Database show
show all sequences of 1.14.13.51

Metabolism of 1,8-cineole by a Rhodococcus species: Ring cleavage reactions

Williams, D.R.; Trudgill, P.W.; Taylor, D.G.; J. Gen. Microbiol. 135, 1957-1967 (1989)
No PubMed abstract available

Data extracted from this reference:

General Stability
General Stability
Organism
5-10 vol% ethanol stabilizes the crude cell extract
Rhodococcus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
6-oxocineole + NADPH + O2
Rhodococcus
grown on 1,8-cineole or succinate as carbon source
1,6,6-trimethyl-2,7-dioxabicyclo-[3.2.2]nonan-3-one + NADP+ + H2O
this product reacts spontaneously to 3-(1-hydroxy-1-methylethyl)-6-oxoheptanoic acid and subsequently lactonizes to 4,5-dihydro-5,5-dimethyl-4-(3'-oxobutyl)-furan-2(3H)-one
Rhodococcus
?
6-oxocineole + NADPH + O2
Rhodococcus C1
grown on 1,8-cineole or succinate as carbon source
1,6,6-trimethyl-2,7-dioxabicyclo-[3.2.2]nonan-3-one + NADP+ + H2O
this product reacts spontaneously to 3-(1-hydroxy-1-methylethyl)-6-oxoheptanoic acid and subsequently lactonizes to 4,5-dihydro-5,5-dimethyl-4-(3'-oxobutyl)-furan-2(3H)-one
Rhodococcus C1
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Rhodococcus
-
-
-
Rhodococcus C1
-
-
-
Purification (Commentary)
Commentary
Organism
partial
Rhodococcus
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.001
-
succinate grown cells
Rhodococcus
0.3
0.4
1,8-cineole-grown cells
Rhodococcus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
6-oxocineole + NADPH + O2
-
286367
Rhodococcus
1,6,6-trimethyl-2,7-dioxabicyclo-[3.2.2]nonan-3-one + NADP+ + H2O
this product reacts spontaneously to 3-(1-hydroxy-1-methylethyl)-6-oxoheptanoic acid and subsequently lactonizes to 4,5-dihydro-5,5-dimethyl-4-(3'-oxobutyl)-furan-2(3H)-one
286367
Rhodococcus
?
6-oxocineole + NADPH + O2
grown on 1,8-cineole or succinate as carbon source
286367
Rhodococcus
1,6,6-trimethyl-2,7-dioxabicyclo-[3.2.2]nonan-3-one + NADP+ + H2O
this product reacts spontaneously to 3-(1-hydroxy-1-methylethyl)-6-oxoheptanoic acid and subsequently lactonizes to 4,5-dihydro-5,5-dimethyl-4-(3'-oxobutyl)-furan-2(3H)-one
286367
Rhodococcus
?
6-oxocineole + NADPH + O2
-
286367
Rhodococcus C1
1,6,6-trimethyl-2,7-dioxabicyclo-[3.2.2]nonan-3-one + NADP+ + H2O
this product reacts spontaneously to 3-(1-hydroxy-1-methylethyl)-6-oxoheptanoic acid and subsequently lactonizes to 4,5-dihydro-5,5-dimethyl-4-(3'-oxobutyl)-furan-2(3H)-one
286367
Rhodococcus C1
?
6-oxocineole + NADPH + O2
grown on 1,8-cineole or succinate as carbon source
286367
Rhodococcus C1
1,6,6-trimethyl-2,7-dioxabicyclo-[3.2.2]nonan-3-one + NADP+ + H2O
this product reacts spontaneously to 3-(1-hydroxy-1-methylethyl)-6-oxoheptanoic acid and subsequently lactonizes to 4,5-dihydro-5,5-dimethyl-4-(3'-oxobutyl)-furan-2(3H)-one
286367
Rhodococcus C1
?
additional information
no substrates: substrate analogues, i.e. (+)-camphor, (S)-(+)-carvone, cyclohexanene, 1,3-cyclohexanedione, dihydrocarvone, 2-, 3-, and 4-methylcyclohexanone, fenchone, menthone, pinan-3-one, pulegone, verbenone
286367
Rhodococcus
?
-
-
-
-
additional information
no substrates: substrate analogues, i.e. (+)-camphor, (S)-(+)-carvone, cyclohexanene, 1,3-cyclohexanedione, dihydrocarvone, 2-, 3-, and 4-methylcyclohexanone, fenchone, menthone, pinan-3-one, pulegone, verbenone
286367
Rhodococcus C1
?
-
-
-
-
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
9
-
-
Rhodococcus
Cofactor
Cofactor
Commentary
Organism
Structure
NADP+
-
Rhodococcus
NADPH
-
Rhodococcus
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADP+
-
Rhodococcus
NADPH
-
Rhodococcus
General Stability (protein specific)
General Stability
Organism
5-10 vol% ethanol stabilizes the crude cell extract
Rhodococcus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
6-oxocineole + NADPH + O2
Rhodococcus
grown on 1,8-cineole or succinate as carbon source
1,6,6-trimethyl-2,7-dioxabicyclo-[3.2.2]nonan-3-one + NADP+ + H2O
this product reacts spontaneously to 3-(1-hydroxy-1-methylethyl)-6-oxoheptanoic acid and subsequently lactonizes to 4,5-dihydro-5,5-dimethyl-4-(3'-oxobutyl)-furan-2(3H)-one
Rhodococcus
?
6-oxocineole + NADPH + O2
Rhodococcus C1
grown on 1,8-cineole or succinate as carbon source
1,6,6-trimethyl-2,7-dioxabicyclo-[3.2.2]nonan-3-one + NADP+ + H2O
this product reacts spontaneously to 3-(1-hydroxy-1-methylethyl)-6-oxoheptanoic acid and subsequently lactonizes to 4,5-dihydro-5,5-dimethyl-4-(3'-oxobutyl)-furan-2(3H)-one
Rhodococcus C1
?
Purification (Commentary) (protein specific)
Commentary
Organism
partial
Rhodococcus
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.001
-
succinate grown cells
Rhodococcus
0.3
0.4
1,8-cineole-grown cells
Rhodococcus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
6-oxocineole + NADPH + O2
-
286367
Rhodococcus
1,6,6-trimethyl-2,7-dioxabicyclo-[3.2.2]nonan-3-one + NADP+ + H2O
this product reacts spontaneously to 3-(1-hydroxy-1-methylethyl)-6-oxoheptanoic acid and subsequently lactonizes to 4,5-dihydro-5,5-dimethyl-4-(3'-oxobutyl)-furan-2(3H)-one
286367
Rhodococcus
?
6-oxocineole + NADPH + O2
grown on 1,8-cineole or succinate as carbon source
286367
Rhodococcus
1,6,6-trimethyl-2,7-dioxabicyclo-[3.2.2]nonan-3-one + NADP+ + H2O
this product reacts spontaneously to 3-(1-hydroxy-1-methylethyl)-6-oxoheptanoic acid and subsequently lactonizes to 4,5-dihydro-5,5-dimethyl-4-(3'-oxobutyl)-furan-2(3H)-one
286367
Rhodococcus
?
6-oxocineole + NADPH + O2
-
286367
Rhodococcus C1
1,6,6-trimethyl-2,7-dioxabicyclo-[3.2.2]nonan-3-one + NADP+ + H2O
this product reacts spontaneously to 3-(1-hydroxy-1-methylethyl)-6-oxoheptanoic acid and subsequently lactonizes to 4,5-dihydro-5,5-dimethyl-4-(3'-oxobutyl)-furan-2(3H)-one
286367
Rhodococcus C1
?
6-oxocineole + NADPH + O2
grown on 1,8-cineole or succinate as carbon source
286367
Rhodococcus C1
1,6,6-trimethyl-2,7-dioxabicyclo-[3.2.2]nonan-3-one + NADP+ + H2O
this product reacts spontaneously to 3-(1-hydroxy-1-methylethyl)-6-oxoheptanoic acid and subsequently lactonizes to 4,5-dihydro-5,5-dimethyl-4-(3'-oxobutyl)-furan-2(3H)-one
286367
Rhodococcus C1
?
additional information
no substrates: substrate analogues, i.e. (+)-camphor, (S)-(+)-carvone, cyclohexanene, 1,3-cyclohexanedione, dihydrocarvone, 2-, 3-, and 4-methylcyclohexanone, fenchone, menthone, pinan-3-one, pulegone, verbenone
286367
Rhodococcus
?
-
-
-
-
additional information
no substrates: substrate analogues, i.e. (+)-camphor, (S)-(+)-carvone, cyclohexanene, 1,3-cyclohexanedione, dihydrocarvone, 2-, 3-, and 4-methylcyclohexanone, fenchone, menthone, pinan-3-one, pulegone, verbenone
286367
Rhodococcus C1
?
-
-
-
-
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
9
-
-
Rhodococcus
Other publictions for EC 1.14.13.51
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
286367
Williams
-
Metabolism of 1,8-cineole by a ...
Rhodococcus, Rhodococcus C1
J. Gen. Microbiol.
135
1957-1967
1989
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1
-
-
-
-
-
2
-
2
-
-
1
-
-
-
2
-
6
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
-
2
-
-
-
1
-
-
2
-
6
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-