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Literature summary for 1.14.13.59 extracted from

  • Thariath, A.; Socha, D.; Valvano, M.A.; Viswanatha, T.
    Construction and biochemical characterization of recombinant cytoplasmic forms of IucD protein (lysine:N6-hydroxylase) encoded by the pColV-K30 aerobactin gene cluster (1993), J. Bacteriol., 175, 589-596.
    View publication on PubMedView publication on EuropePMC
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Protein Variants

Protein Variants Comment Organism
additional information construction of recombinant IucD proteins with modified amino termini by creating three in-frame gene fusions of IucD to the amino-terminal amino acids of the cytoplasmic enzyme beta-galactosidase. Two of these constructs result in the addition of the iucD coding region of a hydrophilic leader sequence of 13 and 30 amino acids. The other construct involves the deletion of the first 47 amino acids of the IucD amino terminus and the addition of 19 amino acids of the amino terminus of beta-galactosidase. Cells expressing any of the three recombinant IucD forms produce soluble N6-hydroxylysine Escherichia coli

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane enzyme from strain EN222 and strain GR143 Escherichia coli 16020
-
soluble recombinant enzyme forms Escherichia coli
-
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-Lys + NADPH + O2 Escherichia coli enzyme catalyzes the first step in aerobactin biosynthesis ?
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
 strain GR143 and EN222, and strain which produce recombinant enzymes
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme form IucD439, in which the sequence encoding the IucD protein is fused in frame to the amino-terminal sequence of beta-galactosidase Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-Lys + NADPH + O2
-
 Escherichia coli N6-Hydroxy-L-Lys + NADP+ + H2O
-
 ?
L-Lys + NADPH + O2 enzyme catalyzes the first step in aerobactin biosynthesis Escherichia coli ?
-
 ?

Cofactor

Cofactor Comment Organism Structure
FAD
-
 Escherichia coli
NADPH required Escherichia coli