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Literature summary for 1.14.13.63 extracted from

  • Suemori, A.; Nakajima, K.; Kurane, R.; Nakamura, Y.
    Purification and characterization of o-hydroxyphenylacetate 5-hydroxylase, m-hydroxyphenylacetate 6-hydroxylase and p-hydroxyphenylacetate 1-hydroxylase from Rhodococcus erythropolis (1996), J. Ferment. Bioeng., 81, 133-137.
No PubMed abstract available

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45000
-
SDS-PAGE Rhodococcus erythropolis
50000
-
gel filtration Rhodococcus erythropolis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3-Hydroxyphenylacetate + NADH + O2 Rhodococcus erythropolis enables growth on 3-hydroxyphenylacetate as sole carbon source 2,5-Dihydroxyphenylacetate + NAD+
-
?
3-Hydroxyphenylacetate + NADH + O2 Rhodococcus erythropolis S1 enables growth on 3-hydroxyphenylacetate as sole carbon source 2,5-Dihydroxyphenylacetate + NAD+
-
?

Organism

Organism UniProt Comment Textmining
Rhodococcus erythropolis
-
highest enzyme activity when grown on 3-hydroxyphenylacetate
-
Rhodococcus erythropolis S1
-
highest enzyme activity when grown on 3-hydroxyphenylacetate
-

Purification (Commentary)

Purification (Comment) Organism
-
Rhodococcus erythropolis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2544
-
purified enzyme, pH 8.0, 30°C Rhodococcus erythropolis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-hydroxyphenoxyacetate + NADH + O2 20% of the activity with 3-hydroxyphenylacetate Rhodococcus erythropolis ?
-
?
2-hydroxyphenoxyacetate + NADH + O2 20% of the activity with 3-hydroxyphenylacetate Rhodococcus erythropolis S1 ?
-
?
3-Hydroxyphenylacetate + NADH + O2
-
Rhodococcus erythropolis 2,5-Dihydroxyphenylacetate + NAD+
-
?
3-Hydroxyphenylacetate + NADH + O2 enables growth on 3-hydroxyphenylacetate as sole carbon source Rhodococcus erythropolis 2,5-Dihydroxyphenylacetate + NAD+
-
?
3-Hydroxyphenylacetate + NADH + O2
-
Rhodococcus erythropolis S1 2,5-Dihydroxyphenylacetate + NAD+
-
?
3-Hydroxyphenylacetate + NADH + O2 enables growth on 3-hydroxyphenylacetate as sole carbon source Rhodococcus erythropolis S1 2,5-Dihydroxyphenylacetate + NAD+
-
?

Subunits

Subunits Comment Organism
monomer 1 * 45000, SDS-PAGE Rhodococcus erythropolis

Cofactor

Cofactor Comment Organism Structure
FAD
-
Rhodococcus erythropolis
additional information no activity with NADPH Rhodococcus erythropolis
NADH
-
Rhodococcus erythropolis