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Literature summary for 1.14.13.7 extracted from
- Evaluation of different phenol hydroxylase-possessing phenol-degrading pseudomonads by kinetic parameters (2008), Biodegradation, 19, 759-769.
Application
| Application | Comment | Organism |
|---|---|---|
| degradation | phenol degradation, among kinetic parameters of growth, the maximum specific growth rate significantly affects the rate of contaminant degradation and is therefore an important parameter to characterise microbes in biological reatment systems | Pseudomonas fluorescens |
| degradation | phenol degradation, among kinetic parameters of growth, the maximum specific growth rate significantly affects the rate of contaminant degradation and is therefore an important parameter to characterise microbes in biological reatment systems | Pseudomonas putida |
| degradation | phenol degradation, among kinetic parameters of growth, the maximum specific growth rate significantly affects the rate of contaminant degradation and is therefore an important parameter to characterise microbes in biological reatment systems | Pseudomonas mendocina |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| DNA and amino acid sequence determination and analysis, expression analysis | Pseudomonas mendocina |
| gene pheBA, DNA and amino acid sequence determination and analysis, expression analysis | Pseudomonas fluorescens |
| gene pheBA, DNA and amino acid sequence determination and analysis, expression analysis | Pseudomonas putida |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| phenol | - |
Pseudomonas fluorescens |  |
| phenol | - |
Pseudomonas mendocina | |
| phenol | - |
Pseudomonas putida | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | kinetic analysis | Pseudomonas mendocina | |
| additional information | - |
additional information | kinetic analysis of the enzyme from different strains, overview | Pseudomonas fluorescens | |
| additional information | - |
additional information | kinetic analysis of the enzyme from different strains, overview | Pseudomonas putida | |
| 0.0013 | - |
phenol | strain B PC30 | Pseudomonas putida | |
| 0.0014 | - |
phenol | strain B PC18 | Pseudomonas fluorescens | |
| 0.0017 | - |
phenol | strain PC1 | Pseudomonas mendocina | |
| 0.0019 | - |
phenol | strain F PC17 | Pseudomonas fluorescens | |
| 0.0024 | - |
phenol | strain B PC16 | Pseudomonas putida | |
| 0.0128 | - |
phenol | strain C PC31 | Pseudomonas fluorescens | |
| 0.0128 | - |
phenol | strain EST1412 | Pseudomonas putida | |
| 0.0131 | - |
phenol | strain F PC20 | Pseudomonas fluorescens | |
| 0.0214 | - |
phenol | strain C PC24 | Pseudomonas fluorescens | |
| 0.0271 | - |
phenol | strain F P69 | Pseudomonas fluorescens | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| phenol + NADH + H+ + O2 | Pseudomonas fluorescens | - |
catechol + NAD+ + H2O | - |
? | |
| phenol + NADH + H+ + O2 | Pseudomonas putida | - |
catechol + NAD+ + H2O | - |
? | |
| phenol + NADH + H+ + O2 | Pseudomonas mendocina | - |
catechol + NAD+ + H2O | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas fluorescens | - |
gene pheBA, single and multicomponent enzyme containing strains C PC24, F PC20, C PC31, F P69, B PC18, and F PC17 | - |
| Pseudomonas mendocina | - |
multicomponent enzyme containing strain PC1 | - |
| Pseudomonas putida | - |
gene pheBA, single and multicomponent enzyme containing strains B PC30, B PC16, and EST1412 | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| phenol + NADH + H+ + O2 | - |
Pseudomonas fluorescens | catechol + NAD+ + H2O | - |
? | |
| phenol + NADH + H+ + O2 | - |
Pseudomonas putida | catechol + NAD+ + H2O | - |
? | |
| phenol + NADH + H+ + O2 | - |
Pseudomonas mendocina | catechol + NAD+ + H2O | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Mph | - |
Pseudomonas fluorescens |
| Mph | - |
Pseudomonas putida |
| Mph | - |
Pseudomonas mendocina |
| multicomponent PH | - |
Pseudomonas fluorescens |
| multicomponent PH | - |
Pseudomonas putida |
| multicomponent PH | - |
Pseudomonas mendocina |
| phenol hydroxylase | - |
Pseudomonas fluorescens |
| phenol hydroxylase | - |
Pseudomonas putida |
| phenol hydroxylase | - |
Pseudomonas mendocina |
| single-component PH | - |
Pseudomonas fluorescens |
| single-component PH | - |
Pseudomonas putida |
| SPH | - |
Pseudomonas fluorescens |
| SPH | - |
Pseudomonas putida |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADH | - |
Pseudomonas fluorescens | |
| NADH | - |
Pseudomonas putida | |
| NADH | - |
Pseudomonas mendocina | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.99 | - |
phenol | strain F P69 | Pseudomonas fluorescens | |
| 2.52 | - |
phenol | strain B PC18 | Pseudomonas fluorescens | |
| 3.44 | - |
phenol | strain F PC20 | Pseudomonas fluorescens | |
| 3.56 | - |
phenol | strain F PC17 | Pseudomonas fluorescens | |
| 3.84 | - |
phenol | strain B PC16 | Pseudomonas putida | |
| 4.31 | - |
phenol | strain C PC31 | Pseudomonas fluorescens | |
| 4.4 | - |
phenol | strain B PC30 | Pseudomonas putida | |
| 7.44 | - |
phenol | strain C PC24 | Pseudomonas fluorescens | |
| 7.57 | - |
phenol | strain EST1412 | Pseudomonas putida | |
| 9.41 | - |
phenol | strain PC1 | Pseudomonas mendocina | |
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