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Literature summary for 1.14.13.7 extracted from

  • Izzo, V.; Leo, G.; Scognamiglio, R.; Troncone, L.; Birolo, L.; Di Donato, A.
    PHK from phenol hydroxylase of Pseudomonas sp. OX1. Insight into the role of an accessory protein in bacterial multicomponent monooxygenases (2011), Arch. Biochem. Biophys., 505, 48-59.
    View publication on PubMed
Search for more literature for 1.14.13.7

Cloned(Commentary)

Cloned (Comment) Organism
expression of the phenol hydroxylase gene cluster in Escherichia coli strain JM109 in the absence or presence of the phk gene Pseudomonas sp.

Inhibitors

Inhibitors Comment Organism Structure
accessory component PHK of phenol hydroxylase the accessory component of the phenol hydroxylase mediates inhibition of phenol hydroxylase activity, overview Pseudomonas sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ PHK, the accessory component of the phenol hydroxylase, may be responsible for enhancing iron incorporation into the active site of the apo-hydroxylase Pseudomonas sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
25500
-
 PHK accessory protein Pseudomonas sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
phenol + NADPH + H+ + O2 Pseudomonas sp.
-
 catechol + NADP+ + H2O
-
 ?
phenol + NADPH + H+ + O2 Pseudomonas sp. OX1
-
 catechol + NADP+ + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Pseudomonas sp.
-
-
-
Pseudomonas sp. OX1
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant PHK, the accessory component of the phenol hydroxylase, from Escherichia coli Pseudomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
phenol + NADPH + H+ + O2
-
 Pseudomonas sp. catechol + NADP+ + H2O
-
 ?
phenol + NADPH + H+ + O2 monitoring the production of catechol in a continuous coupled assay with recombinant catechol 2,3-dioxygenase from Pseudomonas sp. OX1 Pseudomonas sp. catechol + NADP+ + H2O
-
 ?
phenol + NADPH + H+ + O2
-
 Pseudomonas sp. OX1 catechol + NADP+ + H2O
-
 ?
phenol + NADPH + H+ + O2 monitoring the production of catechol in a continuous coupled assay with recombinant catechol 2,3-dioxygenase from Pseudomonas sp. OX1 Pseudomonas sp. OX1 catechol + NADP+ + H2O
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 10235.27, PHK accessory protein, mass spectrometry Pseudomonas sp.
More the phenol hydroxylase complex possesses the PHL, PHN, PHO, PHM, and PHK subunits Pseudomonas sp.

Synonyms

Synonyms Comment Organism
phenol hydroxylase
-
 Pseudomonas sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Pseudomonas sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 assay at Pseudomonas sp.

General Information

General Information Comment Organism
additional information PHK, the accessory component of the phenol hydroxylase, is neither involved in the catalytic activity of the phenol hydroxylase complex nor required for the assembly of apo-hydroxylase, but may be responsible for enhancing iron incorporation into the active site of the apo-hydroxylase Pseudomonas sp.