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Literature summary for 1.14.13.7 extracted from

  • Long, Y.; Yang, S.; Xie, Z.; Cheng, L.
    Identification and characterization of phenol hydroxylase from phenol-degrading Candida tropicalis strain JH8 (2014), Can. J. Microbiol., 60, 585-591 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Candida tropicalis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.21
-
phenol at pH 7.6 and 30°C Candida tropicalis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
320000
-
gel filtration Candida tropicalis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
phenol + NADPH + H+ + O2 Candida tropicalis
-
catechol + NADP+ + H2O
-
?
phenol + NADPH + H+ + O2 Candida tropicalis JH8
-
catechol + NADP+ + H2O
-
?

Organism

Organism UniProt Comment Textmining
Candida tropicalis
-
-
-
Candida tropicalis JH8
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
phenol + NADPH + H+ + O2
-
Candida tropicalis catechol + NADP+ + H2O
-
?
phenol + NADPH + H+ + O2
-
Candida tropicalis JH8 catechol + NADP+ + H2O
-
?

Subunits

Subunits Comment Organism
homotetramer 4 * 80000, SDS-PAGE Candida tropicalis

Synonyms

Synonyms Comment Organism
phenol hydroxylase
-
Candida tropicalis
phhY
-
Candida tropicalis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
Candida tropicalis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
activity is rapidly lost at temperatures above 60°C Candida tropicalis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.6
-
-
Candida tropicalis

Cofactor

Cofactor Comment Organism Structure
NADPH
-
Candida tropicalis