KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0031 | - |
NADPH | with bicyclo[3.2.0]hept-2-en-6-one as cosubstrate, at pH 8.0 and 20°C | Rhodococcus jostii | |
0.01 | - |
NADH | with bicyclo[3.2.0]hept-2-en-6-one as cosubstrate, at pH 8.0 and 20°C | Rhodococcus jostii | |
1 | - |
bicyclo[4.2.0]octan-7-one | at pH 8.0 and 20°C | Rhodococcus jostii | |
2.1 | - |
Cyclobutanone | at pH 8.0 and 20°C | Rhodococcus jostii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodococcus jostii | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Strep-tag affinity column chromatography | Rhodococcus jostii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 bicyclo[4.2.0]octan-7-one + 2 NADH + 2 H+ + 2 O2 | - |
Rhodococcus jostii | (3aS,7aS)-hexahydro-1-benzofuran-2(3H)-one + (3aR,7aS)-hexahydro-2-benzofuran-1(3H)-one + 2 NAD+ + 2 H2O | - |
? | |
bicyclo[3.2.0]hept-2-en-6-one + NADH + H+ + O2 | - |
Rhodococcus jostii | ? | - |
? | |
bicyclo[3.2.0]hept-2-en-6-one + NADPH + H+ + O2 | - |
Rhodococcus jostii | ? | - |
? | |
cyclobutanone + NADH + H+ + O2 | - |
Rhodococcus jostii | gamma-butyrolactone + NAD+ + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
FMO-E | - |
Rhodococcus jostii |
type II flavin-containing monooxygenase | - |
Rhodococcus jostii |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.3 | - |
Cyclobutanone | at pH 8.0 and 20°C | Rhodococcus jostii | |
1.7 | - |
bicyclo[4.2.0]octan-7-one | at pH 8.0 and 20°C | Rhodococcus jostii | |
2 | - |
NADH | with bicyclo[3.2.0]hept-2-en-6-one as cosubstrate, at pH 8.0 and 20°C | Rhodococcus jostii | |
2.8 | - |
NADPH | with bicyclo[3.2.0]hept-2-en-6-one as cosubstrate, at pH 8.0 and 20°C | Rhodococcus jostii |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Rhodococcus jostii | |
NADH | the enzyme accepts both NADPH and NADH as cofactor | Rhodococcus jostii | |
NADPH | the enzyme accepts both NADPH and NADH as cofactor | Rhodococcus jostii |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.5 | - |
Cyclobutanone | at pH 8.0 and 20°C | Rhodococcus jostii | |
0.8 | - |
NADH | with bicyclo[3.2.0]hept-2-en-6-one as cosubstrate, at pH 8.0 and 20°C | Rhodococcus jostii | |
1 | - |
NADPH | with bicyclo[3.2.0]hept-2-en-6-one as cosubstrate, at pH 8.0 and 20°C | Rhodococcus jostii | |
1.7 | - |
bicyclo[4.2.0]octan-7-one | at pH 8.0 and 20°C | Rhodococcus jostii |