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Literature summary for 1.14.13.81 extracted from

  • Rzeznicka, K.; Walker, C.J.; Westergren, T.; Kannangara, C.G.; von Wettstein, D.; Merchant, S.; Gough, S.P.; Hansson, M.
    Xantha-l encodes a membrane subunit of the aerobic Mg-protoporphyrin IX monomethyl ester cyclase involved in chlorophyll biosynthesis (2005), Proc. Natl. Acad. Sci. USA, 102, 5886-5891.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
gene xantha-1, DNA and amino acid sequence determination and analysis, expression of wild-type and mutants in transgenic barley plants Hordeum vulgare

Protein Variants

Protein Variants Comment Organism
additional information construction of xantha-l35, xantha-l81, xantha-l82, viridis-k23, and viridis-k170 mutants, the mutants are all inactive in cyclase activity, phenotypes of mutant plants, overview Hordeum vulgare

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast
-
Hordeum vulgare 9507
-
membrane the aerobic cyclase is composed of three gene products, a soluble protein, a membrane-bound component encoded by xantha-l, and another membrane-bound component encoded by viridis-k Hordeum vulgare 16020
-
soluble the aerobic cyclase is composed of three gene products, a soluble protein, a membrane-bound component encoded by xantha-l, and another membrane-bound component encoded by viridis-k Hordeum vulgare
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Hordeum vulgare

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
magnesium-protoporphyrin IX 13-monomethyl ester + NADPH + H+ + O2 Hordeum vulgare the enzyme is involved in the chlorophyll biosynthesis magnesium-protochlorophyllide + NADP+ + H2O
-
ir

Organism

Organism UniProt Comment Textmining
Hordeum vulgare Q5EFU4 gene xantha-I; cv. Bonus, the aerobic cyclase is composed of three gene products, a soluble protein, a membrane-bound component encoded by xantha-l, and another membrane-bound component encoded by viridis-k
-

Purification (Commentary)

Purification (Comment) Organism
partially by chloroplast supernatant and membrane preparation of wild-type and recombinant mutant plants Hordeum vulgare

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
Hordeum vulgare
-
seedling
-
Hordeum vulgare
-
stem
-
Hordeum vulgare
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0000085
-
average wild-type activity in chloroplasts Hordeum vulgare

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
magnesium-protoporphyrin IX 13-monomethyl ester + NADPH + H+ + O2
-
Hordeum vulgare magnesium-protochlorophyllide + NADP+ + H2O
-
ir
magnesium-protoporphyrin IX 13-monomethyl ester + NADPH + H+ + O2 the enzyme is involved in the chlorophyll biosynthesis Hordeum vulgare magnesium-protochlorophyllide + NADP+ + H2O
-
ir

Subunits

Subunits Comment Organism
More the aerobic cyclase is composed of three different subunits: a soluble protein, a membrane-bound component encoded by Xantha-l, and another membrane-bound component encoded by viridis-k Hordeum vulgare

Synonyms

Synonyms Comment Organism
aerobic Mg-protoporphyrin IX monomethyl ester cyclase
-
Hordeum vulgare

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Hordeum vulgare

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8
-
assay at Hordeum vulgare

Cofactor

Cofactor Comment Organism Structure
NADPH
-
Hordeum vulgare