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Literature summary for extracted from

  • Bang, W.Y.; Jeong, I.S.; Kim, D.W.; Im, C.H.; Ji, C.; Hwang, S.M.; Kim, S.W.; Son, Y.S.; Jeong, J.; Shiina, T.; Bahk, J.D.
    Role of Arabidopsis CHL27 protein for photosynthesis, chloroplast development and gene expression profiling (2008), Plant Cell Physiol., 49, 1350-1363.
    View publication on PubMed


Cloned (Comment) Organism
PCR-amplification of cDNA from isolated RNA, PCR-products of enzyme-GFP fusion constructs are cloned into vector pPENSOTG and introduced into Arabidopsis protoplasts by polyethylene glycol-mediated transformation, complementation constructs are produced by joining PCR fragments of the enzyme promoter with the enzyme-GFP cDNA, cloned into vector pBIB-HYG, introduced into Agrobacterium GV3101, transformed into homozygous mutant plants via the floral dipping method Arabidopsis thaliana

Protein Variants

Protein Variants Comment Organism
additional information mutant plants are complemented with enzyme construct fused to GFP at C-terminus under native promoter or a constitutive 35S-promoter control, suppressing the mutant phenotype, N-terminal deletion of the first 39 amino acids leads to location in the cytosol instead of chloroplast membrane (crucial localization signal) Arabidopsis thaliana


Localization Comment Organism GeneOntology No. Textmining
chloroplast membrane N-terminal 39 amino acids are essential for chloroplast localization of the enzyme Arabidopsis thaliana 31969

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
magnesium-protoporphyrin IX monomethyl ester + NADPH + H+ + O2 Arabidopsis thaliana
protochlorophyllide a + NADP+ + H2O


Organism UniProt Comment Textmining
Arabidopsis thaliana Q9M591 chl27-t knock-down mutant

Source Tissue

Source Tissue Comment Organism Textmining
Arabidopsis thaliana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
magnesium-protoporphyrin IX monomethyl ester + NADPH + H+ + O2
Arabidopsis thaliana protochlorophyllide a + NADP+ + H2O


Synonyms Comment Organism
aerobic Mg-protoporphyrin IX monomethyl ester cyclase
Arabidopsis thaliana
Arabidopsis thaliana

General Information

General Information Comment Organism
malfunction the Arabidopsis chl27-t knock-down mutant has a T-DNA insertion within the promoter region reducing mRNA levels dramatically, growth is slowed down and plants look pale due to chloroplast development defects (unstacking of thylakoid membranes), chlorophyll fluorescence analysis shows a low photosynthetic activity, numerous nuclear genes involved in pigment biosynthesis, photosynthesis (light-harvesting complex I and II, photo system I and II), for iron acquisition, carbon fixation, cold acclimation, and for electron carriers are repressed, stress and detoxifying related gene expression is affected most prominently, more than 600 genes are repressed, a similar number of genes are induced Arabidopsis thaliana
metabolism enzyme is involved in the protochlorophyllide a synthesis Arabidopsis thaliana