Application | Comment | Organism |
---|---|---|
medicine | the enzyme is an attractive target for the treatment of ischemic stroke | Pseudomonas fluorescens |
Cloned (Comment) | Organism |
---|---|
functional expression in Escherichia coli strain BL21 (DE3) | Pseudomonas fluorescens |
General Stability | Organism |
---|---|
disulfide reductants like dithiothreitol or 2-mercaptoethanol stabilize the enzyme | Pseudomonas fluorescens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
3-nitrobenzoylalanine | - |
Pseudomonas fluorescens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | steady-state kinetics and ligand perturbation of flavin fluorescence, overview | Pseudomonas fluorescens | |
0.0085 | - |
NADPH | recombinant enzyme, pH 7.5, 5°C | Pseudomonas fluorescens | |
0.012 | - |
kynurenine | recombinant enzyme, pH 7.5, 5°C | Pseudomonas fluorescens | |
0.034 | - |
O2 | recombinant enzyme, pH 7.5, 5°C | Pseudomonas fluorescens | |
0.053 | - |
kynurenine | recombinant enzyme, pH 7.5, 25°C | Pseudomonas fluorescens | |
0.071 | - |
O2 | recombinant enzyme, pH 7.5, 25°C | Pseudomonas fluorescens | |
0.091 | - |
NADPH | recombinant enzyme, pH 7.5, 25°C | Pseudomonas fluorescens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50000 | - |
x * 50000, recombinant enzyme, SDS-PAGE | Pseudomonas fluorescens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
kynurenine + NADPH + O2 | Pseudomonas fluorescens | the reaction is central to the tryptophan degradative pathway, overview, and takes place within microglial cells defining cellular concentrations of the N-methyl-D-aspatate receptor agonist quinolinate and antagonist kynurenate | 3-hydroxy-kynurenine + NADP+ + H2O | the product acts as apoptotic signal | ir | |
kynurenine + NADPH + O2 | Pseudomonas fluorescens 17400 | the reaction is central to the tryptophan degradative pathway, overview, and takes place within microglial cells defining cellular concentrations of the N-methyl-D-aspatate receptor agonist quinolinate and antagonist kynurenate | 3-hydroxy-kynurenine + NADP+ + H2O | the product acts as apoptotic signal | ir |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas fluorescens | - |
- |
- |
Pseudomonas fluorescens 17400 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme 8.8fold from Escherichia coli strain by ammonium sulfate fractionation and ion exchange chromatography to homogeneity | Pseudomonas fluorescens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
microglia | - |
Pseudomonas fluorescens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
3.15 | - |
purified recombinant enzyme | Pseudomonas fluorescens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
kynurenine + NADPH + O2 | the reaction is central to the tryptophan degradative pathway, overview, and takes place within microglial cells defining cellular concentrations of the N-methyl-D-aspatate receptor agonist quinolinate and antagonist kynurenate | Pseudomonas fluorescens | 3-hydroxy-kynurenine + NADP+ + H2O | the product acts as apoptotic signal | ir | |
kynurenine + NADPH + O2 | enzyme activity depends on the reduction state of the enzyme | Pseudomonas fluorescens | 3-hydroxy-kynurenine + NADP+ + H2O | - |
ir | |
kynurenine + NADPH + O2 | the reaction is central to the tryptophan degradative pathway, overview, and takes place within microglial cells defining cellular concentrations of the N-methyl-D-aspatate receptor agonist quinolinate and antagonist kynurenate | Pseudomonas fluorescens 17400 | 3-hydroxy-kynurenine + NADP+ + H2O | the product acts as apoptotic signal | ir | |
kynurenine + NADPH + O2 | enzyme activity depends on the reduction state of the enzyme | Pseudomonas fluorescens 17400 | 3-hydroxy-kynurenine + NADP+ + H2O | - |
ir |
Subunits | Comment | Organism |
---|---|---|
? | x * 50000, recombinant enzyme, SDS-PAGE | Pseudomonas fluorescens |
Synonyms | Comment | Organism |
---|---|---|
KMO | - |
Pseudomonas fluorescens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Pseudomonas fluorescens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.3 | - |
kynurenine | recombinant enzyme, pH 7.5, 5°C | Pseudomonas fluorescens | |
4.9 | - |
kynurenine | recombinant enzyme, pH 7.5, 25°C | Pseudomonas fluorescens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
- |
Pseudomonas fluorescens |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.2 | 9.7 | - |
Pseudomonas fluorescens |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | 7 | purified recombinant enzyme, 4°C, stable | Pseudomonas fluorescens |
8 | - |
purified recombinant enzyme, 4°C, 10 h, loss of 21% of activity | Pseudomonas fluorescens |
9 | - |
purified recombinant enzyme, 4°C, 10 h, loss of 32% of activity | Pseudomonas fluorescens |
10 | - |
purified recombinant enzyme, 4°C, 3 h, complete inactivation | Pseudomonas fluorescens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | flavoprotein | Pseudomonas fluorescens | |
NADPH | dependent on | Pseudomonas fluorescens |