Crystallization (Comment) | Organism |
---|---|
PAMO crystal structure analysis, comparison to cyclohexanone monooxygenase, EC 1.14.13.22 | Thermobifida fusca |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of three mutants P1-P3 by elimination of a bulge loop region, involving residues Ser441, Ala442, and Leu443, leading to enhanced substrate enantioselectivity of Baeyer-Villiger reactions while maintaining high thermal stability, overview | Thermobifida fusca |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.059 | - |
phenylacetone | wild-type enzyme | Thermobifida fusca | |
0.07 | - |
2-phenylcyclohexanone | mutant P3 | Thermobifida fusca | |
0.5 | - |
2-phenylcyclohexanone | mutant P2 | Thermobifida fusca | |
2.3 | - |
2-phenylcyclohexanone | mutant P1 | Thermobifida fusca | |
2.5 | - |
phenylacetone | mutant P3 | Thermobifida fusca | |
3 | - |
phenylacetone | mutant P1 | Thermobifida fusca | |
4 | - |
phenylacetone | mutant P2 | Thermobifida fusca |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermobifida fusca | - |
a moderately thermophilic bacterium | - |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
phenylacetone + NADPH + H+ + O2 = benzyl acetate + NADP+ + H2O | reaction mechanism | Thermobifida fusca |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Thermobifida fusca |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-methylphenylcyclohexanone + NADPH + O2 | mutant P3 prefers the R-isomer | Thermobifida fusca | 7-benzyloxepan-2-one + NADP+ + H2O | - |
? | |
2-phenylcyclohexanone + NADPH + O2 | molecular modeling of the Criegee intermediate, the wild-type enzyme prefers the S-isomer, while mutants P1-P3 all prefer the R-isomer | Thermobifida fusca | 7-phenyloxepan-2-one + NADP+ + H2O | - |
? | |
4-phenylcyclohexanone + NADPH + O2 | - |
Thermobifida fusca | 4-phenyl-hexano-6-lactone + NADP+ + H2O | - |
? | |
additional information | substrate selectivity and stereospecificity of wild-type and mutant enzymes, overview | Thermobifida fusca | ? | - |
? | |
phenylacetone + NADPH + H+ + O2 | - |
Thermobifida fusca | benzyl acetate + NADP+ + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | structure analysis, the enzyme exhibits a two-domain architecture, and a bulge loop region | Thermobifida fusca |
Synonyms | Comment | Organism |
---|---|---|
More | the enzyme belongs to the Baeyer-Villiger monooxygenases | Thermobifida fusca |
PAMO | - |
Thermobifida fusca |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Thermobifida fusca |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.22 | - |
phenylacetone | mutant P3 | Thermobifida fusca | |
0.25 | - |
phenylacetone | mutant P1 | Thermobifida fusca | |
0.25 | - |
2-phenylcyclohexanone | mutant P3 | Thermobifida fusca | |
0.31 | - |
2-phenylcyclohexanone | mutant P1 | Thermobifida fusca | |
0.4 | - |
phenylacetone | mutant P2 | Thermobifida fusca | |
0.5 | - |
2-phenylcyclohexanone | mutant P2 | Thermobifida fusca | |
1.9 | - |
phenylacetone | wild-type enzyme | Thermobifida fusca |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | binding structure involving Arg337, overview | Thermobifida fusca | |
NADPH | - |
Thermobifida fusca |