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Literature summary for 1.14.13.92 extracted from

  • Rehdorf, J.; Kirschner, A.; Bornscheuer, U.T.
    Cloning, expression and characterization of a Baeyer-Villiger monooxygenase from Pseudomonas putida KT2440 (2007), Biotechnol. Lett., 29, 1393-1398.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
BVMO gene, functional expression in Escherichia coli strains JM109 and BL21(DE3) as soluble protein Pseudomonas putida

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2-decanone + NADPH + O2 Pseudomonas putida
-
? + NADP+ + H2O
-
?
2-decanone + NADPH + O2 Pseudomonas putida KT 2240
-
? + NADP+ + H2O
-
?
2-dodecanone + NADPH + O2 Pseudomonas putida
-
? + NADP+ + H2O
-
?
2-dodecanone + NADPH + O2 Pseudomonas putida KT 2240
-
? + NADP+ + H2O
-
?
2-nonanone + NADPH + O2 Pseudomonas putida
-
? + NADP+ + H2O
-
?
2-nonanone + NADPH + O2 Pseudomonas putida KT 2240
-
? + NADP+ + H2O
-
?
2-undecanone + NADPH + O2 Pseudomonas putida
-
? + NADP+ + H2O
-
?
3-decanone + NADPH + O2 Pseudomonas putida
-
? + NADP+ + H2O
-
?
4-decanone + NADPH + O2 Pseudomonas putida
-
? + NADP+ + H2O
-
?
additional information Pseudomonas putida substrate specificity and reaction mechanism, the enzyme shows high specificity towards short-chain aliphatic ketones, some open-chain ketones are converted to the alkylacetates, while for others formation of the ester products with oxygen on the other side of the keto group can also be detected yielding the corresponding methyl or ethyl esters, overview ?
-
?
additional information Pseudomonas putida KT 2240 substrate specificity and reaction mechanism, the enzyme shows high specificity towards short-chain aliphatic ketones, some open-chain ketones are converted to the alkylacetates, while for others formation of the ester products with oxygen on the other side of the keto group can also be detected yielding the corresponding methyl or ethyl esters, overview ?
-
?

Organism

Organism UniProt Comment Textmining
Pseudomonas putida
-
BVMO gene
-
Pseudomonas putida KT 2240
-
BVMO gene
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-decanone + NADPH + O2
-
Pseudomonas putida ? + NADP+ + H2O
-
?
2-decanone + NADPH + O2
-
Pseudomonas putida KT 2240 ? + NADP+ + H2O
-
?
2-dodecanone + NADPH + O2
-
Pseudomonas putida ? + NADP+ + H2O
-
?
2-dodecanone + NADPH + O2
-
Pseudomonas putida KT 2240 ? + NADP+ + H2O
-
?
2-nonanone + NADPH + O2
-
Pseudomonas putida ? + NADP+ + H2O
-
?
2-nonanone + NADPH + O2
-
Pseudomonas putida KT 2240 ? + NADP+ + H2O
-
?
2-undecanone + NADPH + O2
-
Pseudomonas putida ? + NADP+ + H2O
-
?
3-decanone + NADPH + O2
-
Pseudomonas putida ? + NADP+ + H2O
-
?
4-decanone + NADPH + O2
-
Pseudomonas putida ? + NADP+ + H2O
-
?
4-decanone + NADPH + O2 best substrate Pseudomonas putida ? + NADP+ + H2O
-
?
additional information substrate specificity and reaction mechanism, the enzyme shows high specificity towards short-chain aliphatic ketones, some open-chain ketones are converted to the alkylacetates, while for others formation of the ester products with oxygen on the other side of the keto group can also be detected yielding the corresponding methyl or ethyl esters, overview Pseudomonas putida ?
-
?
additional information the enzyme shows high specificity towards short-chain aliphatic ketones, some open-chain ketones are converted to the alkylacetates, while for others formation of the ester products with oxygen on the other side of the keto group can also be detected yielding the corresponding methyl or ethyl esters, overview, no activity with 2-octanone and 2-tridecanone Pseudomonas putida ?
-
?
additional information substrate specificity and reaction mechanism, the enzyme shows high specificity towards short-chain aliphatic ketones, some open-chain ketones are converted to the alkylacetates, while for others formation of the ester products with oxygen on the other side of the keto group can also be detected yielding the corresponding methyl or ethyl esters, overview Pseudomonas putida KT 2240 ?
-
?
additional information the enzyme shows high specificity towards short-chain aliphatic ketones, some open-chain ketones are converted to the alkylacetates, while for others formation of the ester products with oxygen on the other side of the keto group can also be detected yielding the corresponding methyl or ethyl esters, overview, no activity with 2-octanone and 2-tridecanone Pseudomonas putida KT 2240 ?
-
?

Synonyms

Synonyms Comment Organism
Baeyer-Villiger monooxygenase
-
Pseudomonas putida
BVMO
-
Pseudomonas putida
More the enzyme belongs to the Baeyer-Villiger monooxygenases Pseudomonas putida

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
20 30 biotransformation assay Pseudomonas putida
25
-
assay at Pseudomonas putida

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Pseudomonas putida

Cofactor

Cofactor Comment Organism Structure
NADPH
-
Pseudomonas putida