Application | Comment | Organism |
---|---|---|
synthesis | the enzyme is useful for kinetic resolution of a set of racemic substituted 3-phenylbutan-2-ones and synthesis of enantiopure compounds, overview | Thermobifida fusca |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | effects of a range of solvents on the biocatalytic properties of the biocatalyst, overview | Thermobifida fusca |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
phenylacetone + NADPH + H+ + O2 | Thermobifida fusca | - |
benzyl acetate + NADP+ + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermobifida fusca | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
phenylacetone + NADPH + H+ + O2 = benzyl acetate + NADP+ + H2O | reaction mechanism and catalytic cycle, rapid binding of NADPH is followed by a transfer of the (4R)-hydride from NADPH to the FAD cofactor. The reduced PAMO is rapidly oxygenated by molecular oxygen, yielding a C4a-peroxyflavin. The peroxyflavin enzyme intermediate, possibly a Criegee intermediate or a C4a-hydroxyflavin form, reacts with phenylacetone to form benzylacetate, residue R337 is important in catalysis, overview | Thermobifida fusca |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R)-2-acetoxyphenylacetonitrile + NADPH + H+ + O2 | enantioselective reaction | Thermobifida fusca | ? | - |
? | |
(R)-3-(4-bromophenyl)butan-2-one + NADPH + H+ + O2 | enantioselective reaction | Thermobifida fusca | ? | - |
? | |
(S)-1-(3-trifluoromethylphenyl)ethyl acetate + NADPH + H+ + O2 | enantioselective reaction | Thermobifida fusca | ? | - |
? | |
3-(3-trifluoromethylphenyl)butan-2-one + NADPH + H+ + O2 | enantioselective reaction | Thermobifida fusca | ? | - |
? | |
3-phenyl-2-butanone + NADPH + H+ + O2 | enantioselective reaction | Thermobifida fusca | (R)-3-phenylbutan-2-one + (S)-1-phenyethyl acetate | - |
? | |
alpha-acetylphenylacetonitrile + NADPH + H+ + O2 | enantioselective reaction | Thermobifida fusca | (R)-2-acetoxyphenylacetonitrile + NADP+ + H2O | enantiopure product formation | ? | |
phenylacetone + NADPH + H+ + O2 | - |
Thermobifida fusca | benzyl acetate + NADP+ + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PAMO | - |
Thermobifida fusca |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Thermobifida fusca |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Thermobifida fusca |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Thermobifida fusca |