Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.14.13.92 extracted from

  • Dudek, H.M.; Fink, M.J.; Shivange, A.V.; Dennig, A.; Mihovilovic, M.D.; Schwaneberg, U.; Fraaije, M.W.
    Extending the substrate scope of a Baeyer-Villiger monooxygenase by multiple-site mutagenesis (2013), Appl. Microbiol. Biotechnol., 98, 4009-4020.
    View publication on PubMed
Search for more literature for 1.14.13.92

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Thermobifida fusca

Protein Variants

Protein Variants Comment Organism
P253F/G254A/R258M/L443F the mutant shows the same thermostability as the wild type enzyme while it displays an extended substrate spectrum Thermobifida fusca
W501A the mutant shows reduced activity compared to the wild type enzyme Thermobifida fusca

Inhibitors

Inhibitors Comment Organism Structure
2-Octanone substrate inhibition Thermobifida fusca
phenylacetone substrate inhibition Thermobifida fusca

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.04
-
 phenylacetone mutant enzyme P253F/G254A/R258M/L443F, at pH 7.5 and 37°C Thermobifida fusca
0.08
-
 phenylacetone wild type enzyme, at pH 7.5 and 37°C Thermobifida fusca
0.25
-
 2-Octanone mutant enzyme P253F/G254A/R258M/L443F, at pH 7.5 and 37°C Thermobifida fusca
0.8
-
 2-phenylcyclohexanone mutant enzyme P253F/G254A/R258M/L443F, at pH 7.5 and 37°C Thermobifida fusca
2
-
 2-Octanone wild type enzyme, at pH 7.5 and 37°C Thermobifida fusca
4
-
 2-phenylcyclohexanone wild type enzyme, at pH 7.5 and 37°C Thermobifida fusca
1000
-
 Cyclopentanone mutant enzyme P253F/G254A/R258M/L443F, at pH 7.5 and 37°C Thermobifida fusca
1200
-
 Cyclopentanone wild type enzyme, at pH 7.5 and 37°C Thermobifida fusca

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
phenylacetone + NADPH + H+ + O2 Thermobifida fusca
-
 benzyl acetate + NADP+ + H2O
-
 ?

Organic Solvent Stability

Organic Solvent Comment Organism
additional information the initial activity of wild type enzyme is not affected by 10% (v/v) 1,4-dioxane Thermobifida fusca

Organism

Organism UniProt Comment Textmining
Thermobifida fusca
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2R,3S)-3-methyl-2-pentylcyclopentanone + NADPH + H+ + O2 less than 5% conversion Thermobifida fusca ?
-
 ?
2-benzylcyclopentanone + NADPH + H+ + O2 about 10% conversion Thermobifida fusca ?
-
 ?
2-nonanone + NADPH + H+ + O2 less than 40% conversion Thermobifida fusca ?
-
 ?
2-octanone + NADPH + H+ + O2
-
 Thermobifida fusca ?
-
 ?
2-phenylcyclohexanone + NADPH + H+ + O2
-
 Thermobifida fusca ?
-
 ?
3-(4-chlorophenyl)cyclobutanone + NADPH + H+ + O2 about 40% conversion Thermobifida fusca ?
-
 ?
3-benzylcyclobutanone + NADPH + H+ + O2 about 45% conversion Thermobifida fusca ?
-
 ?
3-octanone + NADPH + H+ + O2
-
 Thermobifida fusca ?
-
 ?
3-phenylcyclobutanone + NADPH + H+ + O2 about 70% conversion Thermobifida fusca ?
-
 ?
bicyclo[2.2.1]heptan-2-one + NADPH + H+ + O2 about 5% conversion Thermobifida fusca ?
-
 ?
bicyclo[3.2.0]hept-2-en-6-one + NADPH + H+ + O2
-
 Thermobifida fusca ?
-
 ?
cyclohexanone + NADPH + H+ + O2
-
 Thermobifida fusca ?
-
 ?
cyclopentanone + NADPH + H+ + O2
-
 Thermobifida fusca ?
-
 ?
phenylacetone + NADPH + H+ + O2
-
 Thermobifida fusca benzyl acetate + NADP+ + H2O
-
 ?
thioanisole + NADPH + H+ + O2
-
 Thermobifida fusca ?
-
 ?

Synonyms

Synonyms Comment Organism
Baeyer-Villiger monooxygenase
-
 Thermobifida fusca
PAMO
-
 Thermobifida fusca

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 the wild type enzyme has a melting temperature of 60°C Thermobifida fusca

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.07
-
 2-phenylcyclohexanone wild type enzyme, at pH 7.5 and 37°C Thermobifida fusca
0.3
-
 2-phenylcyclohexanone mutant enzyme P253F/G254A/R258M/L443F, at pH 7.5 and 37°C Thermobifida fusca
0.9
-
 Cyclopentanone wild type enzyme, at pH 7.5 and 37°C Thermobifida fusca
1
-
 2-Octanone wild type enzyme, at pH 7.5 and 37°C Thermobifida fusca
1.4
-
 phenylacetone mutant enzyme P253F/G254A/R258M/L443F, at pH 7.5 and 37°C Thermobifida fusca
1.6
-
 Cyclopentanone mutant enzyme P253F/G254A/R258M/L443F, at pH 7.5 and 37°C Thermobifida fusca
2.3
-
 2-Octanone mutant enzyme P253F/G254A/R258M/L443F, at pH 7.5 and 37°C Thermobifida fusca
3
-
 phenylacetone wild type enzyme, at pH 7.5 and 37°C Thermobifida fusca

Cofactor

Cofactor Comment Organism Structure
NADPH
-
 Thermobifida fusca

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.008
-
 2-phenylcyclohexanone wild type enzyme, at pH 7.5 and 37°C Thermobifida fusca
0.37
-
 2-phenylcyclohexanone mutant enzyme P253F/G254A/R258M/L443F, at pH 7.5 and 37°C Thermobifida fusca
0.48
-
 2-Octanone wild type enzyme, at pH 7.5 and 37°C Thermobifida fusca
0.8
-
 Cyclopentanone wild type enzyme, at pH 7.5 and 37°C Thermobifida fusca
1.6
-
 Cyclopentanone mutant enzyme P253F/G254A/R258M/L443F, at pH 7.5 and 37°C Thermobifida fusca
9.2
-
 2-Octanone mutant enzyme P253F/G254A/R258M/L443F, at pH 7.5 and 37°C Thermobifida fusca
35
-
 phenylacetone mutant enzyme P253F/G254A/R258M/L443F, at pH 7.5 and 37°C Thermobifida fusca
37.5
-
 phenylacetone wild type enzyme, at pH 7.5 and 37°C Thermobifida fusca